Isolation and compositional analysis of trypanosomatid editosomes

Aswini K Panigrahi, Achim Schnaufer, Kenneth D Stuart

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Most mitochondrial (mt) mRNAs in trypanosomes undergo posttranscriptional RNA editing, which inserts and deletes uridines (Us) to produce the mature and functional mRNA. The editing process is catalyzed by multiple enzymatic steps and is carried out by an approximately 20S macromolecular complex, the editosome. Editosomes have been purified from Trypanosoma brucei using various techniques including combinations of column chromatography, gradient sedimentation, monoclonal antibody affinity, and TAP-tag affinity approaches. This article describes in detail the methods for editosome purification and identification of protein components by mass spectrometry analyses. It also describes the methods for isolation and analysis of TAP-tagged mutagenized complexes.
Original languageEnglish
Pages (from-to)3-24
Number of pages22
JournalMethods in enzymology
Volume424
DOIs
Publication statusPublished - 2007

Fingerprint

Dive into the research topics of 'Isolation and compositional analysis of trypanosomatid editosomes'. Together they form a unique fingerprint.

Cite this