Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

D. C. de Graaf; P. Berghen; L. Moens; T. M. de Marez; S. Raes; M. L. Blaxter; J. Vercruysse

doi:10.1017/S0031182000066282

Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

D. C. de Graaf, P. Berghen, L. Moens, T. M. de Marez, S. Raes, M. L. Blaxter, J. Vercruysse

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Western blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts of Ostertagia ostertagi adults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated molecular mass of 36 kDa under non-reducing conditions, suggesting a dimeric structure containing 2 non-covalently linked 17 kDa monomers. Tryptic peptides were sequenced and showed strong similarities with the globins of free-living and parasitic nematodes. Immunolocalization revealed the presence of this globin-like antigen in the body wall musculature and/or the cuticle of O. ostertagi adults. An enzyme-linked immunosorbent assay based on the purified OoAdGlb showed no differences in response between calves infected by O. ostertagi and/or Cooperia oncophora and the negative controls.

Original language	English
Pages (from-to)	63-69
Journal	Parasitology
Volume	113
Issue number	1
DOIs	https://doi.org/10.1017/S0031182000066282
Publication status	Published - Jul 1996

Keywords

nematode
Ostertagia ostertagi
haemoglobin

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10.1017/S0031182000066282

Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults
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title = "Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults",

abstract = "Western blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts of Ostertagia ostertagi adults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated molecular mass of 36 kDa under non-reducing conditions, suggesting a dimeric structure containing 2 non-covalently linked 17 kDa monomers. Tryptic peptides were sequenced and showed strong similarities with the globins of free-living and parasitic nematodes. Immunolocalization revealed the presence of this globin-like antigen in the body wall musculature and/or the cuticle of O. ostertagi adults. An enzyme-linked immunosorbent assay based on the purified OoAdGlb showed no differences in response between calves infected by O. ostertagi and/or Cooperia oncophora and the negative controls.",

keywords = "nematode, Ostertagia ostertagi, haemoglobin",

author = "{de Graaf}, {D. C.} and P. Berghen and L. Moens and {de Marez}, {T. M.} and S. Raes and Blaxter, {M. L.} and J. Vercruysse",

note = "0031-1820 Journal Article",

year = "1996",

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doi = "10.1017/S0031182000066282",

language = "English",

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T1 - Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

AU - de Graaf, D. C.

AU - Berghen, P.

AU - Moens, L.

AU - de Marez, T. M.

AU - Raes, S.

AU - Blaxter, M. L.

AU - Vercruysse, J.

N1 - 0031-1820 Journal Article

PY - 1996/7

Y1 - 1996/7

N2 - Western blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts of Ostertagia ostertagi adults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated molecular mass of 36 kDa under non-reducing conditions, suggesting a dimeric structure containing 2 non-covalently linked 17 kDa monomers. Tryptic peptides were sequenced and showed strong similarities with the globins of free-living and parasitic nematodes. Immunolocalization revealed the presence of this globin-like antigen in the body wall musculature and/or the cuticle of O. ostertagi adults. An enzyme-linked immunosorbent assay based on the purified OoAdGlb showed no differences in response between calves infected by O. ostertagi and/or Cooperia oncophora and the negative controls.

AB - Western blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts of Ostertagia ostertagi adults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated molecular mass of 36 kDa under non-reducing conditions, suggesting a dimeric structure containing 2 non-covalently linked 17 kDa monomers. Tryptic peptides were sequenced and showed strong similarities with the globins of free-living and parasitic nematodes. Immunolocalization revealed the presence of this globin-like antigen in the body wall musculature and/or the cuticle of O. ostertagi adults. An enzyme-linked immunosorbent assay based on the purified OoAdGlb showed no differences in response between calves infected by O. ostertagi and/or Cooperia oncophora and the negative controls.

KW - nematode

KW - Ostertagia ostertagi

KW - haemoglobin

U2 - 10.1017/S0031182000066282

DO - 10.1017/S0031182000066282

M3 - Article

VL - 113

SP - 63

EP - 69

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 1

ER -

Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

Abstract

Keywords

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