Projects per year
Abstract
Whereas hundreds of cells in the mouse embryonic aorta transdifferentiate to hematopoietic cells, only very few establish hematopoietic stem cell (HSC) identity at a single time point. The Gata2 transcription factor is essential for HSC generation and function. In contrast to surface marker-based cell isolation, Gata2-based enrichment provides a direct link to the internal HSC regulatory network. Here we use iterations of index-sorting of Gata2-expressing intra-aortic hematopoietic cluster (IAHC) cells, single cell transcriptomics and functional analyses to connect HSC identity to specific gene expression.
Gata2-expressing IAHC cells separate into 5 major transcriptomic clusters. Iterative analyses reveal refined CD31, cKit and CD27 phenotypic parameters that associate specific
molecular profiles in one cluster with distinct HSC and multipotent progenitor function. Thus, by iterations of single cell approaches, we identify the transcriptome of the first functional HSCs as they emerge in the mouse embryo and localize them to aortic clusters containing 1-2 cells.
Gata2-expressing IAHC cells separate into 5 major transcriptomic clusters. Iterative analyses reveal refined CD31, cKit and CD27 phenotypic parameters that associate specific
molecular profiles in one cluster with distinct HSC and multipotent progenitor function. Thus, by iterations of single cell approaches, we identify the transcriptome of the first functional HSCs as they emerge in the mouse embryo and localize them to aortic clusters containing 1-2 cells.
Original language | English |
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Article number | 107627 |
Journal | Cell Reports |
Volume | 31 |
Issue number | 6 |
DOIs | |
Publication status | Published - 12 May 2020 |
Keywords
- AGM
- CD27
- Gata2
- development
- embryo
- functional identity
- hematopoietic stem cell
- heterogeneity
- intra-aortic hematopoietic clusters
- single-cell transcriptome
- transplantation
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Projects
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Single cell hematopoietic fate acquisition during embryonic development: To be a stem cell or not?
1/01/20 → 31/12/22
Project: Research
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Profiles
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Elaine Dzierzak
- Deanery of Clinical Sciences - Chair of Haematological Regeneration
- Centre for Inflammation Research
- Edinburgh Haematopoiesis Network
Person: Academic: Research Active