iTRAQ proteomic analysis of extracellular matrix remodeling in aortic valve disease

Tatiana Martin-Rojas, Laura Mourino-Alvarez, Sergio Alonso-Orgaz, Esther Rosello-Lleti, Enrique Calvo, Luis Fernando Lopez-Almodovar, Miguel Rivera, Luis R Padial, Juan Antonio Lopez, Fernando de la Cuesta, Maria G Barderas

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Degenerative aortic stenosis (AS) is the most common worldwide cause of valve replacement. The aortic valve is a thin, complex, layered connective tissue with compartmentalized extracellular matrix (ECM) produced by specialized cell types, which directs blood flow in one direction through the heart. There is evidence suggesting remodeling of such ECM during aortic stenosis development. Thus, a better characterization of the role of ECM proteins in this disease would increase our understanding of the underlying molecular mechanisms. Aortic valve samples were collected from 18 patients which underwent aortic valve replacement (50% males, mean age of 74 years) and 18 normal control valves were obtained from necropsies (40% males, mean age of 69 years). The proteome of the samples was analyzed by 2D-LC MS/MS iTRAQ methodology. The results showed an altered expression of 13 ECM proteins of which 3 (biglycan, periostin, prolargin) were validated by Western blotting and/or SRM analyses. These findings are substantiated by our previous results demonstrating differential ECM protein expression. The present study has demonstrated a differential ECM protein pattern in individuals with AS, therefore supporting previous evidence of a dynamic ECM remodeling in human aortic valves during AS development.

Original languageEnglish
Pages (from-to)17290
JournalScientific Reports
Publication statusPublished - 1 Dec 2015


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