Projects per year
Modular cloning standards based on Golden Gate DNA assembly allow for construction of complex DNA constructs over several rounds of assembly. Despite being reliable and automation-friendly, each standard uses a specific set of vectors, requiring researchers to generate new tool kits for novel hosts and cloning applications. JUMP vectors (Valenzuela-Ortega and French, bioRxiv 799585, 2019) combine the robustness of modular cloning standards with the Standard European Vector Architecture and a flexible design that allows researchers to easily modify the vector backbone via secondary cloning sites. This flexibility allows for JUMP vectors to be used in a wide variety of applications and hosts.
|Title of host publication||DNA Cloning and Assembly|
|Subtitle of host publication||Methods and Protocols|
|Publication status||Published - 19 Aug 2020|
|Name||DNA Cloning and Assembly|
- synthetic biology
- molecular cloning
- DNA assembly
- single-pot assembly
- golden gate cloning
FingerprintDive into the research topics of 'Joint universal modular plasmids: A flexible platform for golden gate assembly in any microbial host'. Together they form a unique fingerprint.
- 1 Finished
EASTBIO: EASTBIO East of Scotland BioScience Doctoral Training Partnership studentship - Research Training Grant BB/J01446X/1
1/09/12 → 30/09/18
Project: Other (Non-Funded/Miscellaneous)