Joint universal modular plasmids: A flexible platform for golden gate assembly in any microbial host

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Modular cloning standards based on Golden Gate DNA assembly allow for construction of complex DNA constructs over several rounds of assembly. Despite being reliable and automation-friendly, each standard uses a specific set of vectors, requiring researchers to generate new tool kits for novel hosts and cloning applications. JUMP vectors (Valenzuela-Ortega and French, bioRxiv 799585, 2019) combine the robustness of modular cloning standards with the Standard European Vector Architecture and a flexible design that allows researchers to easily modify the vector backbone via secondary cloning sites. This flexibility allows for JUMP vectors to be used in a wide variety of applications and hosts.
Original languageEnglish
Title of host publicationDNA Cloning and Assembly
Subtitle of host publicationMethods and Protocols
PublisherSpringer
Chapter15
Pages255-273
ISBN (Electronic)978-1-0716-0908-8
ISBN (Print)978-1-0716-0907-1
DOIs
Publication statusPublished - 19 Aug 2020

Publication series

NameDNA Cloning and Assembly
Volume2205
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • synthetic biology
  • molecular cloning
  • DNA assembly
  • single-pot assembly
  • golden gate cloning
  • PhytoBricks
  • JUMP

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