Joint universal modular plasmids: A flexible platform for golden gate assembly in any microbial host

Marcos Valenzuela-Ortega; Christopher E. French

doi:10.1007/978-1-0716-0908-8_15

Joint universal modular plasmids: A flexible platform for golden gate assembly in any microbial host

Marcos Valenzuela-Ortega, Christopher E. French

School of Biological Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract / Description of output

Modular cloning standards based on Golden Gate DNA assembly allow for construction of complex DNA constructs over several rounds of assembly. Despite being reliable and automation-friendly, each standard uses a specific set of vectors, requiring researchers to generate new tool kits for novel hosts and cloning applications. JUMP vectors (Valenzuela-Ortega and French, bioRxiv 799585, 2019) combine the robustness of modular cloning standards with the Standard European Vector Architecture and a flexible design that allows researchers to easily modify the vector backbone via secondary cloning sites. This flexibility allows for JUMP vectors to be used in a wide variety of applications and hosts.

Original language	English
Title of host publication	DNA Cloning and Assembly
Subtitle of host publication	Methods and Protocols
Publisher	Springer
Chapter	15
Pages	255-273
ISBN (Electronic)	978-1-0716-0908-8
ISBN (Print)	978-1-0716-0907-1
DOIs	https://doi.org/10.1007/978-1-0716-0908-8_15
Publication status	Published - 19 Aug 2020

Publication series

Name	DNA Cloning and Assembly
Volume	2205
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords / Materials (for Non-textual outputs)

synthetic biology
molecular cloning
DNA assembly
single-pot assembly
golden gate cloning
PhytoBricks
JUMP

Access to Document

10.1007/978-1-0716-0908-8_15

EASTBIO: EASTBIO East of Scotland BioScience Doctoral Training Partnership studentship - Research Training Grant BB/J01446X/1
Smith, S.
1/09/12 → 30/09/18
Project: Other (Non-Funded/Miscellaneous)

Cite this

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title = "Joint universal modular plasmids: A flexible platform for golden gate assembly in any microbial host",

abstract = "Modular cloning standards based on Golden Gate DNA assembly allow for construction of complex DNA constructs over several rounds of assembly. Despite being reliable and automation-friendly, each standard uses a specific set of vectors, requiring researchers to generate new tool kits for novel hosts and cloning applications. JUMP vectors (Valenzuela-Ortega and French, bioRxiv 799585, 2019) combine the robustness of modular cloning standards with the Standard European Vector Architecture and a flexible design that allows researchers to easily modify the vector backbone via secondary cloning sites. This flexibility allows for JUMP vectors to be used in a wide variety of applications and hosts.",

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doi = "10.1007/978-1-0716-0908-8_15",

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T2 - A flexible platform for golden gate assembly in any microbial host

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AU - French, Christopher E.

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Y1 - 2020/8/19

N2 - Modular cloning standards based on Golden Gate DNA assembly allow for construction of complex DNA constructs over several rounds of assembly. Despite being reliable and automation-friendly, each standard uses a specific set of vectors, requiring researchers to generate new tool kits for novel hosts and cloning applications. JUMP vectors (Valenzuela-Ortega and French, bioRxiv 799585, 2019) combine the robustness of modular cloning standards with the Standard European Vector Architecture and a flexible design that allows researchers to easily modify the vector backbone via secondary cloning sites. This flexibility allows for JUMP vectors to be used in a wide variety of applications and hosts.

AB - Modular cloning standards based on Golden Gate DNA assembly allow for construction of complex DNA constructs over several rounds of assembly. Despite being reliable and automation-friendly, each standard uses a specific set of vectors, requiring researchers to generate new tool kits for novel hosts and cloning applications. JUMP vectors (Valenzuela-Ortega and French, bioRxiv 799585, 2019) combine the robustness of modular cloning standards with the Standard European Vector Architecture and a flexible design that allows researchers to easily modify the vector backbone via secondary cloning sites. This flexibility allows for JUMP vectors to be used in a wide variety of applications and hosts.

KW - synthetic biology

KW - molecular cloning

KW - DNA assembly

KW - single-pot assembly

KW - golden gate cloning

KW - PhytoBricks

KW - JUMP

U2 - 10.1007/978-1-0716-0908-8_15

DO - 10.1007/978-1-0716-0908-8_15

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SN - 978-1-0716-0907-1

T3 - DNA Cloning and Assembly

SP - 255

EP - 273

BT - DNA Cloning and Assembly

PB - Springer

ER -

Joint universal modular plasmids: A flexible platform for golden gate assembly in any microbial host

Abstract / Description of output

Publication series

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Projects

EASTBIO: EASTBIO East of Scotland BioScience Doctoral Training Partnership studentship - Research Training Grant BB/J01446X/1

Cite this