Joint universal modular plasmids (JUMP): A flexible vector platform for synthetic biology

Research output: Contribution to journalArticlepeer-review

Abstract

Generation of new DNA constructs is an essential process in modern life science and biotechnology. Modular cloning systems based on Golden Gate cloning, using Type IIS restriction endonucleases, allow assembly of complex multi-part constructs from re-usable basic DNA parts in a rapid, reliable, and automation-friendly way. Many such toolkits are available, with varying degrees of compatibility, most of which are aimed at specific host organisms. Here we present a vector design which allows simple vector modification by using modular cloning to assemble and add new functions in secondary sites flanking the main insertion site (used for conventional modular cloning). Assembly in all sites is compatible with the PhytoBricks standard, and vectors are compatible with the Standard European Vector Architecture (SEVA) as well as BioBricks. We demonstrate that this facilitates the construction of vectors with tailored functions and simplifies the workflow for generating libraries of constructs with common elements. We have made available a collection of vectors with ten different microbial replication origins, varying in copy number and host range, and allowing chromosomal integration, as well as a selection of commonly used basic parts. This design expands the range of hosts which can be easily modified by modular cloning and acts as a toolkit which can be used to facilitate the generation of new toolkits with specific functions required for targeting further hosts.
Original languageEnglish
Article numberysab003
Number of pages11
JournalSynthetic Biology
Volume6
Issue number1
DOIs
Publication statusPublished - 2 Feb 2021

Keywords

  • synthetic biology
  • DNA assembly
  • golden gate cloning
  • modular cloning
  • SEVA

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