Kalirin and CHD7: Novel endothelial dysfunction indicators in circulating extracellular vesicles from hypertensive patients with albuminuria

Fernando de la Cuesta, Montserrat Baldan-Martin, Rafael Moreno-Luna, Gloria Alvarez-Llamas, Laura Gonzalez-Calero, Laura Mourino-Alvarez, Tamara Sastre-Oliva, Juan A. López, Jesús Vázquez, Gema Ruiz-Hurtado, Julian Segura, Fernando Vivanco, Luis M. Ruilope, Maria G. Barderas*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Despite of the great advances in anti-hypertensive therapies, many patients under Renin-Angiotensin- System (RAS) suppression develop albuminuria, which is a clear indicator of therapeutic inefficiency. Hence, indicators of vascular function are needed to assess patients' condition and help deciding future therapies. Proteomic analysis of circulating extracellular vesicles (EVs) showed two proteins, kalirin and chromodomain-helicase-DNA-binding protein 7 (CHD7), increased in albuminuric patients. A positive correlation of both with the expression of the endothelial activation marker E-selectin was found in EVs. In vitro analysis using TNFa-treated adult human endothelial cells proved their involvement in endothelial cell activation. Hence, we propose protein levels of kalirin and CHD7 in circulating EVs as novel endothelial dysfunction markers to monitor vascular condition in hypertensive patients with albuminuria.

Original languageEnglish
Pages (from-to)15553-15562
Number of pages10
JournalOncotarget
Volume8
Issue number9
DOIs
Publication statusPublished - 1 Feb 2017

Keywords

  • Albuminuria
  • Endothelial dysfunction
  • Extracellular vesicles
  • Hypertension
  • Proteomics

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