TY - JOUR
T1 - Kalirin and CHD7
T2 - Novel endothelial dysfunction indicators in circulating extracellular vesicles from hypertensive patients with albuminuria
AU - de la Cuesta, Fernando
AU - Baldan-Martin, Montserrat
AU - Moreno-Luna, Rafael
AU - Alvarez-Llamas, Gloria
AU - Gonzalez-Calero, Laura
AU - Mourino-Alvarez, Laura
AU - Sastre-Oliva, Tamara
AU - López, Juan A.
AU - Vázquez, Jesús
AU - Ruiz-Hurtado, Gema
AU - Segura, Julian
AU - Vivanco, Fernando
AU - Ruilope, Luis M.
AU - Barderas, Maria G.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Despite of the great advances in anti-hypertensive therapies, many patients under Renin-Angiotensin- System (RAS) suppression develop albuminuria, which is a clear indicator of therapeutic inefficiency. Hence, indicators of vascular function are needed to assess patients' condition and help deciding future therapies. Proteomic analysis of circulating extracellular vesicles (EVs) showed two proteins, kalirin and chromodomain-helicase-DNA-binding protein 7 (CHD7), increased in albuminuric patients. A positive correlation of both with the expression of the endothelial activation marker E-selectin was found in EVs. In vitro analysis using TNFa-treated adult human endothelial cells proved their involvement in endothelial cell activation. Hence, we propose protein levels of kalirin and CHD7 in circulating EVs as novel endothelial dysfunction markers to monitor vascular condition in hypertensive patients with albuminuria.
AB - Despite of the great advances in anti-hypertensive therapies, many patients under Renin-Angiotensin- System (RAS) suppression develop albuminuria, which is a clear indicator of therapeutic inefficiency. Hence, indicators of vascular function are needed to assess patients' condition and help deciding future therapies. Proteomic analysis of circulating extracellular vesicles (EVs) showed two proteins, kalirin and chromodomain-helicase-DNA-binding protein 7 (CHD7), increased in albuminuric patients. A positive correlation of both with the expression of the endothelial activation marker E-selectin was found in EVs. In vitro analysis using TNFa-treated adult human endothelial cells proved their involvement in endothelial cell activation. Hence, we propose protein levels of kalirin and CHD7 in circulating EVs as novel endothelial dysfunction markers to monitor vascular condition in hypertensive patients with albuminuria.
KW - Albuminuria
KW - Endothelial dysfunction
KW - Extracellular vesicles
KW - Hypertension
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=85014109308&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.14948
DO - 10.18632/oncotarget.14948
M3 - Article
AN - SCOPUS:85014109308
VL - 8
SP - 15553
EP - 15562
JO - Oncotarget
JF - Oncotarget
SN - 1949-2553
IS - 9
ER -