Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery

Daniel G. Booth; Masatoshi Takagi; Luis Sanchez-Pulido; Elizabeth Petfalski; Giulia Vargiu; Kumiko Samejima; Naoko Imamoto; Chris P. Ponting; David Tollervey; William C. Earnshaw; Paola Vagnarelli

doi:10.7554/eLife.01641.001

Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery

Daniel G. Booth, Masatoshi Takagi, Luis Sanchez-Pulido, Elizabeth Petfalski, Giulia Vargiu, Kumiko Samejima, Naoko Imamoto, Chris P. Ponting, David Tollervey, William C. Earnshaw, Paola Vagnarelli

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

When the nucleolus disassembles during open mitosis, many nucleolar proteins and RNAs associate with chromosomes, establishing a perichromosomal compartment coating the chromosome periphery. At present nothing is known about the function of this poorly characterised compartment. In this study, we report that the nucleolar protein Ki-67 is required for the assembly of the perichromosomal compartment in human cells. Ki-67 is a cell-cycle regulated protein phosphatase 1-binding protein that is involved in phospho-regulation of the nucleolar protein B23/nucleophosmin. Following siRNA depletion of Ki-67, NIFK, B23, nucleolin, and four novel chromosome periphery proteins all fail to associate with the periphery of human chromosomes. Correlative light and electron microscopy (CLEM) images suggest a near-complete loss of the entire perichromosomal compartment. Mitotic chromosome condensation and intrinsic structure appear normal in the absence of the perichromosomal compartment but significant differences in nucleolar reassembly and nuclear organisation are observed in post-mitotic cells.

Original language	English
Article number	e01641
Number of pages	22
Journal	eLIFE
Volume	3
DOIs	https://doi.org/10.7554/eLife.01641.001
Publication status	Published - 27 May 2014

Keywords / Materials (for Non-textual outputs)

cell nucleolus
chromosomes
HeLa Cells
humans
intracellular signaling peptides and Proteins
Ki-67 Antigen
microscopy
mitosis
molecular sequence data
nuclear proteins
phosphoproteins
phosphorylation
protein phosphatase 1
RNA Interference
RNA-binding proteins
sequence analysis
signal transduction
transfection
electron

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10.7554/eLife.01641.001Licence: Creative Commons: Attribution (CC-BY)

Ki-67 is a PP1-interacting protein that orgainises the mitotic chromosome periphery
Copyright Booth et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Final published version, 4.23 MBLicence: Creative Commons: Attribution (CC-BY)

Core funding renewal for the Wellcome Trust Centre for Cell Biology
Tollervey, D. & Earnshaw, B.
UK-based charities
1/10/11 → 30/04/17
Project: Research
The role of non-histone proteins in chromosome structure and function during mitosis
Earnshaw, B.
UK-based charities
1/01/11 → 30/09/16
Project: Research
Proteomics at the Wellcome Trust Centre for Cell Biology
Rappsilber, J., Tollervey, D. & Tyers, M.
UK-based charities
1/05/10 → 30/04/15
Project: Research

Bill Earnshaw
- School of Biological Sciences - Professor of Chromosome Dynamics
- Centre for Engineering Biology
Person: Academic: Research Active

Cite this

@article{96d053ab020a46a2adc7eae2846e678a,

title = "Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery",

abstract = "When the nucleolus disassembles during open mitosis, many nucleolar proteins and RNAs associate with chromosomes, establishing a perichromosomal compartment coating the chromosome periphery. At present nothing is known about the function of this poorly characterised compartment. In this study, we report that the nucleolar protein Ki-67 is required for the assembly of the perichromosomal compartment in human cells. Ki-67 is a cell-cycle regulated protein phosphatase 1-binding protein that is involved in phospho-regulation of the nucleolar protein B23/nucleophosmin. Following siRNA depletion of Ki-67, NIFK, B23, nucleolin, and four novel chromosome periphery proteins all fail to associate with the periphery of human chromosomes. Correlative light and electron microscopy (CLEM) images suggest a near-complete loss of the entire perichromosomal compartment. Mitotic chromosome condensation and intrinsic structure appear normal in the absence of the perichromosomal compartment but significant differences in nucleolar reassembly and nuclear organisation are observed in post-mitotic cells.",

keywords = "cell nucleolus, chromosomes, HeLa Cells, humans, intracellular signaling peptides and Proteins, Ki-67 Antigen, microscopy, mitosis, molecular sequence data, nuclear proteins, phosphoproteins, phosphorylation, protein phosphatase 1, RNA Interference, RNA-binding proteins, sequence analysis, signal transduction, transfection, electron",

author = "Booth, {Daniel G.} and Masatoshi Takagi and Luis Sanchez-Pulido and Elizabeth Petfalski and Giulia Vargiu and Kumiko Samejima and Naoko Imamoto and Ponting, {Chris P.} and David Tollervey and Earnshaw, {William C.} and Paola Vagnarelli",

year = "2014",

month = may,

day = "27",

doi = "10.7554/eLife.01641.001",

language = "English",

volume = "3",

journal = "eLIFE",

issn = "2050-084X",

publisher = "ELIFE SCIENCES PUBLICATIONS LTD",

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T1 - Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery

AU - Booth, Daniel G.

AU - Takagi, Masatoshi

AU - Sanchez-Pulido, Luis

AU - Petfalski, Elizabeth

AU - Vargiu, Giulia

AU - Samejima, Kumiko

AU - Imamoto, Naoko

AU - Ponting, Chris P.

AU - Tollervey, David

AU - Earnshaw, William C.

AU - Vagnarelli, Paola

PY - 2014/5/27

Y1 - 2014/5/27

N2 - When the nucleolus disassembles during open mitosis, many nucleolar proteins and RNAs associate with chromosomes, establishing a perichromosomal compartment coating the chromosome periphery. At present nothing is known about the function of this poorly characterised compartment. In this study, we report that the nucleolar protein Ki-67 is required for the assembly of the perichromosomal compartment in human cells. Ki-67 is a cell-cycle regulated protein phosphatase 1-binding protein that is involved in phospho-regulation of the nucleolar protein B23/nucleophosmin. Following siRNA depletion of Ki-67, NIFK, B23, nucleolin, and four novel chromosome periphery proteins all fail to associate with the periphery of human chromosomes. Correlative light and electron microscopy (CLEM) images suggest a near-complete loss of the entire perichromosomal compartment. Mitotic chromosome condensation and intrinsic structure appear normal in the absence of the perichromosomal compartment but significant differences in nucleolar reassembly and nuclear organisation are observed in post-mitotic cells.

AB - When the nucleolus disassembles during open mitosis, many nucleolar proteins and RNAs associate with chromosomes, establishing a perichromosomal compartment coating the chromosome periphery. At present nothing is known about the function of this poorly characterised compartment. In this study, we report that the nucleolar protein Ki-67 is required for the assembly of the perichromosomal compartment in human cells. Ki-67 is a cell-cycle regulated protein phosphatase 1-binding protein that is involved in phospho-regulation of the nucleolar protein B23/nucleophosmin. Following siRNA depletion of Ki-67, NIFK, B23, nucleolin, and four novel chromosome periphery proteins all fail to associate with the periphery of human chromosomes. Correlative light and electron microscopy (CLEM) images suggest a near-complete loss of the entire perichromosomal compartment. Mitotic chromosome condensation and intrinsic structure appear normal in the absence of the perichromosomal compartment but significant differences in nucleolar reassembly and nuclear organisation are observed in post-mitotic cells.

KW - cell nucleolus

KW - chromosomes

KW - HeLa Cells

KW - humans

KW - intracellular signaling peptides and Proteins

KW - Ki-67 Antigen

KW - microscopy

KW - mitosis

KW - molecular sequence data

KW - nuclear proteins

KW - phosphoproteins

KW - phosphorylation

KW - protein phosphatase 1

KW - RNA Interference

KW - RNA-binding proteins

KW - sequence analysis

KW - signal transduction

KW - transfection

KW - electron

U2 - 10.7554/eLife.01641.001

DO - 10.7554/eLife.01641.001

M3 - Article

C2 - 24867636

AN - SCOPUS:84902327035

SN - 2050-084X

VL - 3

JO - eLIFE

JF - eLIFE

M1 - e01641

ER -

Ki-67 is a PP1-interacting protein that organises the mitotic chromosome periphery

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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Projects

Core funding renewal for the Wellcome Trust Centre for Cell Biology

The role of non-histone proteins in chromosome structure and function during mitosis

Proteomics at the Wellcome Trust Centre for Cell Biology

Profiles

Bill Earnshaw

Cite this