Projects per year
RNA-binding proteins play a key role in shaping gene expression profiles during stress, however, little is known about the dynamic nature of these interactions and how this influences the kinetics of gene expression. To address this, we developed CRAC, a UV cross-linking method that enabled us to quantitatively measure the dynamics of protein-RNA interactions in vivo on a minute time-scale. Here, using CRAC we measure the global RNA-binding dynamics of the yeast transcription termination factor Nab3 in response to glucose starvation. These measurements reveal rapid changes in protein-RNA interactions within one minute following stress imposition. Changes in Nab3 binding are largely independent of alterations in transcription rate during the early stages of stress response, indicating orthogonal transcriptional control mechanisms. We also uncover a function for Nab3 in dampening expression of stress-responsive genes. CRAC has the potential to greatly enhance our understanding of in vivo dynamics of protein-RNA interactions.
|Publication status||Published - 11 Apr 2017|
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- 6 Finished
Future-Proofing the sustainability of the MRC high throughput sequencing hub in Scotland
1/10/12 → 30/09/14
MLCS - Machine learning for computational science statistical and formal modeling of biological systems
1/10/12 → 30/09/17
Watson, M., Archibald, A. & Burt, D.
1/04/12 → 31/03/17
- School of Biological Sciences - Reader in RNA Biochemistry
- Centre for Engineering Biology
Person: Academic: Research Active