Knocking (Anti)-Sense into Cells: The Microsphere Approach to Gene Silencing

Lois M. Alexander, Rosario M. Sanchez-Martin, Mark Bradley

Research output: Contribution to journalArticlepeer-review

Abstract

200 and 500 nm polymeric microspheres have been conjugated to siRNA targeted against EGFP expressed in human cervical cancer (HeLa) cells and shown to efficiently silence protein expression over 72 h, without detrimental cytotoxicity. Furthermore, with use of an independent Cy5 tracking label on the siRNA-laden microsphere, silencing of EGFP could be assessed by selecting only those cells that contained the delivery vehicle (and thus the siRNA) generating a more accurate picture of microsphere-induced gene silencing.

Original languageEnglish
Pages (from-to)422-426
Number of pages5
JournalBioconjugate chemistry
Volume20
Issue number3
DOIs
Publication statusPublished - Mar 2009

Keywords

  • RNA INTERFERENCE
  • IN-VIVO
  • SIRNA
  • DELIVERY

Cite this