Projects per year
Abstract
200 and 500 nm polymeric microspheres have been conjugated to siRNA targeted against EGFP expressed in human cervical cancer (HeLa) cells and shown to efficiently silence protein expression over 72 h, without detrimental cytotoxicity. Furthermore, with use of an independent Cy5 tracking label on the siRNA-laden microsphere, silencing of EGFP could be assessed by selecting only those cells that contained the delivery vehicle (and thus the siRNA) generating a more accurate picture of microsphere-induced gene silencing.
Original language | English |
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Pages (from-to) | 422-426 |
Number of pages | 5 |
Journal | Bioconjugate chemistry |
Volume | 20 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2009 |
Keywords / Materials (for Non-textual outputs)
- RNA INTERFERENCE
- IN-VIVO
- SIRNA
- DELIVERY
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Dive into the research topics of 'Knocking (Anti)-Sense into Cells: The Microsphere Approach to Gene Silencing'. Together they form a unique fingerprint.Projects
- 1 Finished
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Platform: High- Throughput chemical biology for efficient cellular delivery
Bradley, M.
1/09/05 → 31/08/10
Project: Research