TY - JOUR
T1 - KOPS
T2 - DNA motifs that control E. coli chromosome segregation by orienting the FtsK translocase
AU - Bigot, Sarah
AU - Saleh, Omar A
AU - Lesterlin, Christian
AU - Pages, Carine
AU - El Karoui, Meriem
AU - Dennis, Cynthia
AU - Grigoriev, Mikhail
AU - Allemand, Jean-François
AU - Barre, François-Xavier
AU - Cornet, François
PY - 2005
Y1 - 2005
N2 - Bacterial chromosomes are organized in replichores of opposite sequence polarity. This conserved feature suggests a role in chromosome dynamics. Indeed, sequence polarity controls resolution of chromosome dimers in Escherichia coli. Chromosome dimers form by homologous recombination between sister chromosomes. They are resolved by the combined action of two tyrosine recombinases, XerC and XerD, acting at a specific chromosomal site, dif, and a DNA translocase, FtsK, which is anchored at the division septum and sorts chromosomal DNA to daughter cells. Evidences suggest that DNA motifs oriented from the replication origin towards dif provide FtsK with the necessary information to faithfully distribute chromosomal DNA to either side of the septum, thereby bringing the dif sites together at the end of this process. However, the nature of the DNA motifs acting as FtsK orienting polar sequences (KOPS) was unknown. Using genetics, bioinformatics and biochemistry, we have identified a family of DNA motifs in the E. coli chromosome with KOPS activity.
AB - Bacterial chromosomes are organized in replichores of opposite sequence polarity. This conserved feature suggests a role in chromosome dynamics. Indeed, sequence polarity controls resolution of chromosome dimers in Escherichia coli. Chromosome dimers form by homologous recombination between sister chromosomes. They are resolved by the combined action of two tyrosine recombinases, XerC and XerD, acting at a specific chromosomal site, dif, and a DNA translocase, FtsK, which is anchored at the division septum and sorts chromosomal DNA to daughter cells. Evidences suggest that DNA motifs oriented from the replication origin towards dif provide FtsK with the necessary information to faithfully distribute chromosomal DNA to either side of the septum, thereby bringing the dif sites together at the end of this process. However, the nature of the DNA motifs acting as FtsK orienting polar sequences (KOPS) was unknown. Using genetics, bioinformatics and biochemistry, we have identified a family of DNA motifs in the E. coli chromosome with KOPS activity.
KW - chromosome segregation
KW - FtsK translocase
KW - replichores
U2 - 10.1038/sj.emboj.7600835
DO - 10.1038/sj.emboj.7600835
M3 - Article
C2 - 16211009
SN - 0261-4189
VL - 24
SP - 3770
EP - 3780
JO - EMBO Journal
JF - EMBO Journal
IS - 21
ER -