L-Penicillamine is a mechanism-based inhibitor of serine palmitoyltransferase by forming a pyridoxal-5 '-phosphate-thiazolidine adduct

Jonathan Lowther, Ashley E. Beattie, Pat R. R. Langridge-Smith, David J. Clarke, Dominic J. Campopiano

Research output: Contribution to journalArticlepeer-review

Abstract

Serine palmitoyltransferase (SPT) catalyses the first committed step of de novo sphingolipid biosynthesis. The bacterial SPT homologue from Sphingomonas paucimobilis is a homodimeric enzyme that contains an essential pyridoxal-5'-phosphate (PLP) cofactor bound to each subunit. Inhibitors of SPT are useful blockers of sphingolipid biosynthesis. Here we use UV-vis spectroscopy, enzyme kinetics and mass spectrometry to investigate inhibition of SPT by penicillamine (Pen), a drug with a range of useful medicinal applications.

Original languageEnglish
Pages (from-to)1003-1008
Number of pages6
JournalMedchemcomm
Volume3
Issue number8
DOIs
Publication statusPublished - Aug 2012

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