Laser microdissection and saturation labeling DIGE method for the analysis of human arteries

Fernando de la Cuesta; Gloria Alvarez-Llamas; Aroa S Maroto; Maria G Barderas; Fernando Vivanco

doi:10.1007/978-1-62703-405-0_2

Laser microdissection and saturation labeling DIGE method for the analysis of human arteries

Fernando de la Cuesta, Gloria Alvarez-Llamas, Aroa S Maroto, Maria G Barderas, Fernando Vivanco

Research output: Contribution to journal › Article › peer-review

Abstract

Laser microdissection (LMD) is a novel methodology for noncontact isolation of tissue regions or cells for subsequent molecular analysis. Although it is an upcoming field, its combination with proteomics for differential analysis remains not very well explored, since amount of protein obtained after LMD is scarce. We have combined LMD arterial layer isolation with saturation labeling DIGE, successfully achieving differential analysis of healthy and pathological intima and media layers. Identification of differential spots could be performed in whole tissue extract as reference proteome, since studied regions are subproteomes of the aforementioned.

Original language	English
Pages (from-to)	21-32
Number of pages	12
Journal	Methods in Molecular Biology
Volume	1000
DOIs	https://doi.org/10.1007/978-1-62703-405-0_2
Publication status	Published - 2013

Keywords

Apoptosis
Arteries
Blood Coagulation
Coronary Artery Disease
Extracellular Matrix
Heat-Shock Response
Hemorrhage
Humans
Laser Capture Microdissection
Staining and Labeling
Tandem Mass Spectrometry
Tunica Intima
Tunica Media
Two-Dimensional Difference Gel Electrophoresis

Access to Document

10.1007/978-1-62703-405-0_2

Cite this

@article{356e1dfcce0a4bb09b916124c5a68c83,

title = "Laser microdissection and saturation labeling DIGE method for the analysis of human arteries",

abstract = "Laser microdissection (LMD) is a novel methodology for noncontact isolation of tissue regions or cells for subsequent molecular analysis. Although it is an upcoming field, its combination with proteomics for differential analysis remains not very well explored, since amount of protein obtained after LMD is scarce. We have combined LMD arterial layer isolation with saturation labeling DIGE, successfully achieving differential analysis of healthy and pathological intima and media layers. Identification of differential spots could be performed in whole tissue extract as reference proteome, since studied regions are subproteomes of the aforementioned.",

keywords = "Apoptosis, Arteries, Blood Coagulation, Coronary Artery Disease, Extracellular Matrix, Heat-Shock Response, Hemorrhage, Humans, Laser Capture Microdissection, Staining and Labeling, Tandem Mass Spectrometry, Tunica Intima, Tunica Media, Two-Dimensional Difference Gel Electrophoresis",

author = "{de la Cuesta}, Fernando and Gloria Alvarez-Llamas and Maroto, {Aroa S} and Barderas, {Maria G} and Fernando Vivanco",

year = "2013",

doi = "10.1007/978-1-62703-405-0_2",

language = "English",

volume = "1000",

pages = "21--32",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Laser microdissection and saturation labeling DIGE method for the analysis of human arteries

AU - de la Cuesta, Fernando

AU - Alvarez-Llamas, Gloria

AU - Maroto, Aroa S

AU - Barderas, Maria G

AU - Vivanco, Fernando

PY - 2013

Y1 - 2013

N2 - Laser microdissection (LMD) is a novel methodology for noncontact isolation of tissue regions or cells for subsequent molecular analysis. Although it is an upcoming field, its combination with proteomics for differential analysis remains not very well explored, since amount of protein obtained after LMD is scarce. We have combined LMD arterial layer isolation with saturation labeling DIGE, successfully achieving differential analysis of healthy and pathological intima and media layers. Identification of differential spots could be performed in whole tissue extract as reference proteome, since studied regions are subproteomes of the aforementioned.

AB - Laser microdissection (LMD) is a novel methodology for noncontact isolation of tissue regions or cells for subsequent molecular analysis. Although it is an upcoming field, its combination with proteomics for differential analysis remains not very well explored, since amount of protein obtained after LMD is scarce. We have combined LMD arterial layer isolation with saturation labeling DIGE, successfully achieving differential analysis of healthy and pathological intima and media layers. Identification of differential spots could be performed in whole tissue extract as reference proteome, since studied regions are subproteomes of the aforementioned.

KW - Apoptosis

KW - Arteries

KW - Blood Coagulation

KW - Coronary Artery Disease

KW - Extracellular Matrix

KW - Heat-Shock Response

KW - Hemorrhage

KW - Humans

KW - Laser Capture Microdissection

KW - Staining and Labeling

KW - Tandem Mass Spectrometry

KW - Tunica Intima

KW - Tunica Media

KW - Two-Dimensional Difference Gel Electrophoresis

U2 - 10.1007/978-1-62703-405-0_2

DO - 10.1007/978-1-62703-405-0_2

M3 - Article

C2 - 23585081

VL - 1000

SP - 21

EP - 32

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

Laser microdissection and saturation labeling DIGE method for the analysis of human arteries

Abstract

Keywords

Access to Document

Fingerprint

Cite this