Laser microdissection and saturation labeling DIGE method for the analysis of human arteries

Fernando de la Cuesta, Gloria Alvarez-Llamas, Aroa S Maroto, Maria G Barderas, Fernando Vivanco

Research output: Contribution to journalArticlepeer-review

Abstract

Laser microdissection (LMD) is a novel methodology for noncontact isolation of tissue regions or cells for subsequent molecular analysis. Although it is an upcoming field, its combination with proteomics for differential analysis remains not very well explored, since amount of protein obtained after LMD is scarce. We have combined LMD arterial layer isolation with saturation labeling DIGE, successfully achieving differential analysis of healthy and pathological intima and media layers. Identification of differential spots could be performed in whole tissue extract as reference proteome, since studied regions are subproteomes of the aforementioned.

Original languageEnglish
Pages (from-to)21-32
Number of pages12
JournalMethods in Molecular Biology
Volume1000
DOIs
Publication statusPublished - 2013

Keywords

  • Apoptosis
  • Arteries
  • Blood Coagulation
  • Coronary Artery Disease
  • Extracellular Matrix
  • Heat-Shock Response
  • Hemorrhage
  • Humans
  • Laser Capture Microdissection
  • Staining and Labeling
  • Tandem Mass Spectrometry
  • Tunica Intima
  • Tunica Media
  • Two-Dimensional Difference Gel Electrophoresis

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