Lawsonia intracellularis regulates nuclear factor-κB signalling pathway during infection

Huan W Yang, Tuanjun Hu, Tahar Ait-Ali

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Lawsonia intracellularis is the etiological agent of proliferative enteropathy (PE) in pigs, horses and wide range of mammals. Little is known about the role of innate immune response during L. intracellularis infection. In this study, we investigated the nuclear factor-κB (NF-κB)-regulated immune response against infection of a clinical strain Dkp23 and a live-attenuated Enterisol vaccine strain in PK-15 cells. We found that expression of NF-κB target genes TNF-α, IFN-γ, IL-6 and IL-8 were modulated during the course of infection. At 5 dpi, there was a significant increase in p65 NF-κB activation, including protein nuclear translocation and phosphorylation, synchronous with the induction of IL-6, IFN-γ and IL-8 expression in L. intracellularis infected cells, especially for Enterisol vaccine strain-infected cells. This result suggests that NF-κB signalling level is induced when L. intracellularis bacterial load peaks at 5 dpi. The induction of pro-inflammatory cytokines expression is consistent with the decreased viability of L. intracellularis-infected cells especially that of the vaccine strain. There were no significant changes in NF-κB signalling between vaccine and Dkp23 infection in PK-15 cells, except for moderate levels of differences in NF-κB target genes expression which might be a reflection of differences in intracellular bacterial load. Overall, the data presented here indicate a correlation between the induction of NF-κB signalling and the L. intracellularis bacterial load in PK-15 cells.

Original languageEnglish
Article numbere0310804
Pages (from-to)1-17
Number of pages17
JournalPLOS ONE
Volume19
Issue number9
Early online date26 Sept 2024
DOIs
Publication statusPublished - Sept 2024

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Lawsonia Bacteria/immunology
  • Signal Transduction
  • NF-kappa B/metabolism
  • Swine
  • Cell Line
  • Desulfovibrionaceae Infections/microbiology
  • Cytokines/metabolism
  • Swine Diseases/microbiology
  • Gene Expression Regulation

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