Leaving no-one behind: How CENP-E facilitates chromosome alignment

Benjamin Craske, Julie Welburn

Research output: Contribution to journalReview articlepeer-review

Abstract

Chromosome alignment and biorientation is essential for mitotic progression and genomic
stability. Most chromosomes align at the spindle equator in a motor-independent manner.
However, a subset of polar kinetochores fail to bi-orient and require a microtubule motorbased
transport mechanism to move to the cell equator. Centromere Protein E (CENPE/
KIF10) is a kinesin motor from the Kinesin-7 family, which localises to unattached
kinetochores during mitosis and utilizes plus-end directed microtubule motility to slide monooriented
chromosomes to the spindle equator. Recent work has revealed how CENP-E
cooperates with chromokinesins and dynein to mediate chromosome congression and
highlighted its role at aligned chromosomes. Additionally, we have gained new mechanistic
insights into the targeting and regulation of CENP-E motor activity at the kinetochore. Here,
we will review the function of CENP-E in chromosome congression, the pathways which
contribute to CENP-E loading at the kinetochore, and how CENP-E activity is regulated
during mitosis.
Original languageEnglish
Article numberEBC20190073
Number of pages12
JournalEssays in biochemistry
DOIs
Publication statusPublished - 29 Apr 2020

Keywords

  • CENPE-E
  • congression
  • kinetochore
  • mitosis
  • microtubule
  • kinesin

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