Length-dependent retention of carbon nanotubes in the pleural space of mice initiates sustained inflammation and progressive fibrosis on the parietal pleura

Fiona A Murphy, Craig A Poland, Rodger Duffin, Khuloud T Al-Jamal, Hanene Ali-Boucetta, Antonio Nunes, Fiona Byrne, Adriele Prina-Mello, Yuri Volkov, Shouping Li, Stephen J Mather, Alberto Bianco, Maurizio Prato, William Macnee, William Wallace, Kostas Kostarelos, Ken Donaldson

Research output: Contribution to journalArticlepeer-review

Abstract

The fibrous shape of carbon nanotubes (CNTs) raises concern that they may pose an asbestos-like inhalation hazard, leading to the development of diseases, especially mesothelioma. Direct instillation of long and short CNTs into the pleural cavity, the site of mesothelioma development, produced asbestos-like length-dependent responses. The response to long CNTs and long asbestos was characterized by acute inflammation, leading to progressive fibrosis on the parietal pleura, where stomata of strictly defined size limit the egress of long, but not short, fibers. This was confirmed by demonstrating clearance of short, but not long, CNT and nickel nanowires and by visualizing the migration of short CNTs from the pleural space by single-photon emission computed tomographic imaging. Our data confirm the hypothesis that, although a proportion of all deposited particles passes through the pleura, the pathogenicity of long CNTs and other fibers arises as a result of length-dependent retention at the stomata on the parietal pleura.
Original languageEnglish
Pages (from-to)2587-600
Number of pages14
JournalAmerican Journal of Pathology
Volume178
Issue number6
DOIs
Publication statusPublished - 2011

Keywords

  • Animals
  • Cell Proliferation
  • DISEASE PROGRESSION
  • EPITHELIUM
  • FIBROSIS
  • INFLAMMATION
  • Lymph Nodes
  • mediastinum
  • MICE
  • nanotubes, carbon
  • NANOWIRES
  • Particle Size
  • Pleura
  • Pleural Cavity
  • Time Factors
  • Tomography, Emission-Computed, Single-Photon
  • Tomography, X-Ray Computed

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