Lgr5(+) gastric stem cells divide symmetrically to effect epithelial homeostasis in the pylorus

Marc Leushacke, Annie Ng, Joerg Galle, Markus Loeffler, Nick Barker

Research output: Contribution to journalArticlepeer-review

Abstract

The pyloric epithelium continuously self-renews throughout life, driven by limited reservoirs of resident Lgr5+ adult stem cells. Here, we characterize the population dynamics of these stem cells during epithelial homeostasis. Using a clonal fate-mapping strategy, we demonstrate that multiple Lgr5+ cells routinely contribute to epithelial renewal in the pyloric gland and, similar to what was previously observed in the intestine, a balanced homeostasis of the glandular epithelium and stem cell pools is predominantly achieved via neutral competition between symmetrically dividing Lgr5+ stem cells. Additionally, we document a lateral expansion of stem cell clones via gland fission under nondamage conditions. These findings represent a major advance in our basic understanding of tissue homeostasis in the stomach and form the foundation for identifying altered stem cell behavior during gastric disease.
Original languageEnglish
Pages (from-to)349-56
Number of pages8
JournalCell Reports
Volume5
Issue number2
DOIs
Publication statusPublished - 31 Oct 2013

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