Lgr5(+) gastric stem cells divide symmetrically to effect epithelial homeostasis in the pylorus

Marc Leushacke; Annie Ng; Joerg Galle; Markus Loeffler; Nick Barker

doi:10.1016/j.celrep.2013.09.025

Lgr5(+) gastric stem cells divide symmetrically to effect epithelial homeostasis in the pylorus

Marc Leushacke, Annie Ng, Joerg Galle, Markus Loeffler, Nick Barker

Research output: Contribution to journal › Article › peer-review

Abstract

The pyloric epithelium continuously self-renews throughout life, driven by limited reservoirs of resident Lgr5+ adult stem cells. Here, we characterize the population dynamics of these stem cells during epithelial homeostasis. Using a clonal fate-mapping strategy, we demonstrate that multiple Lgr5+ cells routinely contribute to epithelial renewal in the pyloric gland and, similar to what was previously observed in the intestine, a balanced homeostasis of the glandular epithelium and stem cell pools is predominantly achieved via neutral competition between symmetrically dividing Lgr5+ stem cells. Additionally, we document a lateral expansion of stem cell clones via gland fission under nondamage conditions. These findings represent a major advance in our basic understanding of tissue homeostasis in the stomach and form the foundation for identifying altered stem cell behavior during gastric disease.

Original language	English
Pages (from-to)	349-56
Number of pages	8
Journal	Cell Reports
Volume	5
Issue number	2
DOIs	https://doi.org/10.1016/j.celrep.2013.09.025
Publication status	Published - 31 Oct 2013

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10.1016/j.celrep.2013.09.025

Lgr5(+) gastric stem cells divide symmetrically to effect epithelial homeostasis in the pylorus
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http://www.sciencedirect.com/science/article/pii/S2211124713005482#

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title = "Lgr5(+) gastric stem cells divide symmetrically to effect epithelial homeostasis in the pylorus",

abstract = "The pyloric epithelium continuously self-renews throughout life, driven by limited reservoirs of resident Lgr5+ adult stem cells. Here, we characterize the population dynamics of these stem cells during epithelial homeostasis. Using a clonal fate-mapping strategy, we demonstrate that multiple Lgr5+ cells routinely contribute to epithelial renewal in the pyloric gland and, similar to what was previously observed in the intestine, a balanced homeostasis of the glandular epithelium and stem cell pools is predominantly achieved via neutral competition between symmetrically dividing Lgr5+ stem cells. Additionally, we document a lateral expansion of stem cell clones via gland fission under nondamage conditions. These findings represent a major advance in our basic understanding of tissue homeostasis in the stomach and form the foundation for identifying altered stem cell behavior during gastric disease.",

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AU - Galle, Joerg

AU - Loeffler, Markus

AU - Barker, Nick

PY - 2013/10/31

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AB - The pyloric epithelium continuously self-renews throughout life, driven by limited reservoirs of resident Lgr5+ adult stem cells. Here, we characterize the population dynamics of these stem cells during epithelial homeostasis. Using a clonal fate-mapping strategy, we demonstrate that multiple Lgr5+ cells routinely contribute to epithelial renewal in the pyloric gland and, similar to what was previously observed in the intestine, a balanced homeostasis of the glandular epithelium and stem cell pools is predominantly achieved via neutral competition between symmetrically dividing Lgr5+ stem cells. Additionally, we document a lateral expansion of stem cell clones via gland fission under nondamage conditions. These findings represent a major advance in our basic understanding of tissue homeostasis in the stomach and form the foundation for identifying altered stem cell behavior during gastric disease.

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Lgr5(+) gastric stem cells divide symmetrically to effect epithelial homeostasis in the pylorus

Abstract

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