Limitations of the murine nose in the development of nonviral airway gene transfer

U. Griesenbach, S. G. Sumner-Jones, E. Holder, F. M. Munkonge, T. Wodehouse, S. N. Smith, M. Y. Wasowicz, I. Pringle, I. Casamayor, M. Chan, R. Coles, N. Cornish, A. Dewar, A. Doherty, R. Farley, A. M. Green, B. L. Jones, M. D. Larsen, A. E. Lawton, M. ManvellH. Painter, C. Singh, L. Somerton, B. Stevenson, A. Varathalingam, C. Siegel, R. K. Scheule, S. H. Cheng, J. C. Davies, D. J. Porteous, D. R. Gill, A. C. Boyd, S. C. Hyde, E. W. Alton

Research output: Contribution to journalArticlepeer-review

Abstract

A clinical program to assess whether lipid GL67A-mediated gene transfer can ameliorate cystic fibrosis (CF) lung disease is currently being undertaken by the UK CF Gene Therapy Consortium. We have evaluated GL67A gene transfer to the murine nasal epithelium of wild-type and CF knockout mice to assess this tissue as a test site for gene transfer agents. The plasmids used were regulated by either (1) the commonly used short-acting cytomegalovirus promoter/enhancer or (2) the ubiquitin C promoter. In a study of approximately 400 mice with CF, vector-specific CF transmembrane conductance regulator (CFTR) mRNA was detected in nasal epithelial cells of 82% of mice treated with a cytomegalovirus-plasmid (pCF1-CFTR), and 62% of mice treated with an ubiquitin C-plasmid. We then assessed whether CFTR gene transfer corrected a panel of CFTR-specific endpoint assays in the murine nose, including ion transport, periciliary liquid height, and ex vivo bacterial adherence. Importantly, even with the comparatively large number of animals assessed, the CFTR function studies were only powered to detect changes of more than 50% toward wild-type values. Within this limitation, no significant correction of the CF phenotype was detected. At the current levels of gene transfer efficiency achievable with nonviral vectors, the murine nose is of limited value as a stepping stone to human trials.
Original languageEnglish
Pages (from-to)46-54
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume43
Issue number1
DOIs
Publication statusPublished - 2010

Keywords

  • Animals Bacterial Adhesion Cystic Fibrosis/genetics Cytomegalovirus/genetics Enhancer Elements, Genetic Female Gene Therapy/methods *Gene Transfer Techniques Liposomes/metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Mutation Nose/*pathology Plasmids/metabolism Promoter Regions, Genetic

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