Abstract
We have used a cDNA probe for mouse Gf-1 gene that encodes the erythroid cell transcription factor to identify genetic variation in genomic DNA between Mus species. The segregation of Gf-1 DNA variation was analyzed in Mus species crosses that have been previously typed for the segregation of more than 30 genes spanning 80 cM of the mouse X chromosome from the centromere to the border of the X-Y pairing region. We identified a single X chromosome locus in the mouse, Gf-1, and an analysis of recombinants from 203 backcross progeny mapped Gf-1 to the proximal portion of the chromosome, coincident with the Cybb locus and proximal to Otc gene locus. A gene order of centromere, DXWas70, Cybb/Gf-1, Otc, Timp was established for the mouse X chromosome, which is in agreement with the map position observed on the human X chromosome.
| Original language | English |
|---|---|
| Pages (from-to) | 309-13 |
| Number of pages | 5 |
| Journal | Genomics |
| Volume | 9 |
| Issue number | 2 |
| Publication status | Published - Feb 1991 |
Keywords
- Animals
- Blotting, Southern
- Crosses, Genetic
- DNA-Binding Proteins
- Erythroid-Specific DNA-Binding Factors
- Female
- Genetic Linkage
- Genetic Variation
- Male
- Mice
- Recombination, Genetic
- Transcription Factors
- X Chromosome