Linkage of the erythroid transcription factor gene (Gf-1) to the proximal region of the X chromosome of mice

V M Chapman, D A Stephenson, L J Mullins, B T Keitz, C Disteche, S H Orkin

Research output: Contribution to journalArticlepeer-review

Abstract

We have used a cDNA probe for mouse Gf-1 gene that encodes the erythroid cell transcription factor to identify genetic variation in genomic DNA between Mus species. The segregation of Gf-1 DNA variation was analyzed in Mus species crosses that have been previously typed for the segregation of more than 30 genes spanning 80 cM of the mouse X chromosome from the centromere to the border of the X-Y pairing region. We identified a single X chromosome locus in the mouse, Gf-1, and an analysis of recombinants from 203 backcross progeny mapped Gf-1 to the proximal portion of the chromosome, coincident with the Cybb locus and proximal to Otc gene locus. A gene order of centromere, DXWas70, Cybb/Gf-1, Otc, Timp was established for the mouse X chromosome, which is in agreement with the map position observed on the human X chromosome.

Original languageEnglish
Pages (from-to)309-13
Number of pages5
JournalGenomics
Volume9
Issue number2
Publication statusPublished - Feb 1991

Keywords

  • Animals
  • Blotting, Southern
  • Crosses, Genetic
  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • Female
  • Genetic Linkage
  • Genetic Variation
  • Male
  • Mice
  • Recombination, Genetic
  • Transcription Factors
  • X Chromosome

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