Projects per year
Abstract
Apoptosis of cells and their subsequent removal via efferocytosis occurs in nearly all tissues during development, homeostasis, and disease. However, it has been difficult to track cell death and subsequent corpse removal in vivo. Here, we developed a genetically encoded fluorescent reporter, CharON, that could track emerging apoptotic cells and their efferocytic clearance by phagocytes. Using Drosophila expressing CharON, we uncovered multiple qualitative and quantitative features of coordinated clearance of apoptotic corpses during embryonic development. To confront high rate of emerging apoptotic corpses, the macrophages displayed heterogeneity in engulfment, with some efferocytic macrophages carrying high corpse burden. However, overburdened macrophages were compromised in clearing wound debris, revealing an inherent vulnerability. These findings reveal known and unexpected features of apoptosis and macrophage efferocytosis in vivo.
Original language | English |
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Pages (from-to) | 1182-1187 |
Journal | Science |
Volume | 375 |
Issue number | 6585 |
DOIs | |
Publication status | Published - 11 Mar 2022 |
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Dive into the research topics of 'Live cell tracking of macrophage efferocytosis during Drosophila embryo development in vivo'. Together they form a unique fingerprint.Projects
- 3 Finished
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CIR Sir Henry Wellcome Postdoctoral Fellowship, `Investigating immune cell chemotaxis by using Chromophore Assisted Light Inactivation (CALI) to manipulate actin regulators in real-time.
Wood, W. (Principal Investigator) & Davidson, A. (Co-investigator)
1/09/18 → 1/10/19
Project: Research
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Drosophilia as a model to study immune cell signal integration in vivo
Wood, W. (Principal Investigator)
1/02/18 → 1/12/22
Project: Research
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Macrophage-epithelial communication promotes lung repair after injury
Lucas, C. (Principal Investigator)
3/07/17 → 4/08/22
Project: Research