Live pigs produced from genome edited zygotes

Simon Lillico; Christopher Proudfoot; D. F. Carlson; Dana Stverakova; Claire Neil; Carol Blain; Tim King; WA Ritchie; Spring Tan; A Mileham; D Mclaren; Scott C Fahrenkrug; Bruce Whitelaw

doi:10.1038/srep02847

Live pigs produced from genome edited zygotes

Simon Lillico, Christopher Proudfoot, D. F. Carlson, Dana Stverakova, Claire Neil, Carol Blain, Tim King, WA Ritchie, Spring Tan, A Mileham, D Mclaren, Scott C Fahrenkrug, Bruce Whitelaw

Research output: Contribution to journal › Article › peer-review

Abstract

Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.

Original language	English
Article number	2847
Pages (from-to)	1-4
Journal	Scientific Reports
Volume	3
DOIs	https://doi.org/10.1038/srep02847
Publication status	Published - 2013

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10.1038/srep02847

Live pigs produced from genome edited zygotes
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
Final published version, 363 KBLicence: Creative Commons: Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

Refining genome editing in livestock
Whitelaw, B. (Principal Investigator)
Non-EU industry, commerce and public corporations
30/04/14 → 29/04/16
Project: Research
Evaluation of genome engineering strategies to target the porcine RELA gene
Whitelaw, B. (Principal Investigator)
Non-EU industry, commerce and public corporations
1/01/13 → 31/07/16
Project: Research
Basic mechanisms underlying species-specific trypanosome resistance
Archibald, A. (Principal Investigator), King, T. (Co-investigator) & Whitelaw, B. (Co-investigator)
Non-EU industry, commerce and public corporations
1/05/10 → 30/04/15
Project: Research

Cite this

@article{c723f087ac5c422e8072fd96d481f987,

title = "Live pigs produced from genome edited zygotes",

abstract = "Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.",

author = "Simon Lillico and Christopher Proudfoot and Carlson, {D. F.} and Dana Stverakova and Claire Neil and Carol Blain and Tim King and WA Ritchie and Spring Tan and A Mileham and D Mclaren and Fahrenkrug, {Scott C} and Bruce Whitelaw",

note = "This work was supported by Genus plc, Recombinetics Inc and through BBSRC Institute Stratregic Programme Grant; DS was supported by a Genus plc internship at The Roslin Institute; CP was supported by NSF IOS 0965413.",

year = "2013",

doi = "10.1038/srep02847",

language = "English",

volume = "3",

pages = "1--4",

journal = "Scientific Reports",

issn = "2045-2322",

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T1 - Live pigs produced from genome edited zygotes

AU - Lillico, Simon

AU - Proudfoot, Christopher

AU - Carlson, D. F.

AU - Stverakova, Dana

AU - Neil, Claire

AU - Blain, Carol

AU - King, Tim

AU - Ritchie, WA

AU - Tan, Spring

AU - Mileham, A

AU - Mclaren, D

AU - Fahrenkrug, Scott C

AU - Whitelaw, Bruce

N1 - This work was supported by Genus plc, Recombinetics Inc and through BBSRC Institute Stratregic Programme Grant; DS was supported by a Genus plc internship at The Roslin Institute; CP was supported by NSF IOS 0965413.

PY - 2013

Y1 - 2013

N2 - Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.

AB - Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.

U2 - 10.1038/srep02847

DO - 10.1038/srep02847

M3 - Article

AN - SCOPUS:84879447539

SN - 2045-2322

VL - 3

SP - 1

EP - 4

JO - Scientific Reports

JF - Scientific Reports

M1 - 2847

ER -

Live pigs produced from genome edited zygotes

Abstract

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Projects

Refining genome editing in livestock

Evaluation of genome engineering strategies to target the porcine RELA gene

Basic mechanisms underlying species-specific trypanosome resistance

Cite this