Live‐cell super‐resolution imaging of actin using LifeAct‐14 with a PAINT‐based approach

Haresh Bhaskar, Dirk‐Jan Kleinjan, Curran Oi, Zoe Gidden, Susan J. Rosser, Mathew H. Horrocks, Lynne Regan

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We present direct-LIVE-PAINT, an easy-to-implement approach for the nanoscopic imaging of protein structures in live cells using labeled binding peptides. We demonstrate the feasibility of direct-LIVE-PAINT with an actin-binding peptide fused to EGFP, the location of which can be accurately determined as it transiently binds to actin filaments. We show that direct-LIVE-PAINT can be used to image actin structures below the diffraction-limit of light and have used it to observe the dynamic nature of actin in live cells. We envisage a similar approach could be applied to imaging other proteins within live mammalian cells.
Original languageEnglish
Article numbere4558
Number of pages8
JournalProtein Science
Issue number2
Early online date31 Dec 2022
Publication statusPublished - 1 Feb 2023

Keywords / Materials (for Non-textual outputs)

  • super-resolution (SR) imaging
  • single-molecule localization microscopy (SMLM)
  • actin
  • live-cell imaging
  • LifeAct


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