Local tissue factor pathway inhibitor release in the human forearm

Michiel J B Kemme, Jacobus Burggraaf, Rik C Schoemaker, Adam F Cohen, Cornelis Kluft, Stanley Chia, David J Webb, David E Newby

Research output: Contribution to journalArticlepeer-review


Nineteen healthy men received unilateral brachial artery infusions of either unfractioned heparin (0.3-100 IU/min), saline or the endothelium-dependent vasodilators substance P (2-8 pmol/min) and bradykinin (100-1000 pmol/min), and the endothelium-independent vasodilator sodium nitroprusside (2-8 micro g/min). Heparin caused a dose-dependent increase in plasma TFPI concentrations in both arms (ANOVA, p <0.0001). Estimated net forearm TFPI release was 7 +/- 16, 29 +/- 20 and 138 +/- 72 ng/100 mL tissue/min during 10, 30 and 100 IU/min of heparin respectively (ANOVA, p <0.0001). Compared to the systemic circulation, the forearm sensitivity to heparin induced TFPI release was 3.6-fold lower (166 +/- 67 ng/IU vs. 596 +/- 252 ng/IU: t-test, p = 0.004). Substance P, bradykinin and sodium nitroprusside all caused substantial dose-dependent increases in blood flow (ANOVA, p <0.001 for all) without affecting plasma TFPI concentrations. There are important regional differences in endothelial TFPI release, with the forearm circulation being relatively insensitive to heparin.

Original languageEnglish
Pages (from-to)438-45
Number of pages8
JournalThrombosis and Haemostasis
Issue number3
Publication statusPublished - Mar 2003


  • Adult
  • Bradykinin
  • Endothelium, Vascular
  • Forearm
  • Hemodynamics
  • Heparin
  • Humans
  • Infusions, Intra-Arterial
  • Lipoproteins
  • Male
  • Nitroprusside
  • Regional Blood Flow
  • Substance P
  • Vasodilator Agents


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