Long-lasting effect on the embryogenesis and Igf2 expression following in vitro ethanol exposure during preimplantation

Teodora Taseva, Yana Koycheva, Nelly Olova*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Ethanol is a strong teratogen that affects negatively embryonal and placental growth and development. If administered in vitro during preimplantation, however, it may have a stimulatory effect on the embryogenesis within a narrow range of concentrations. We have previously shown that a dose of 0.2% ethanol in the culture medium stimulates the development of blastocysts and increases the expression levels of the imprinted gene Igf2 [1]. Here we have examined whether the stimulatory effect and altered gene expression are retained through the middle of gestation. Blastocysts from C57BL/6 mice were exposed to 0.2% (v:v) ethanol for 4 h at 3.5 dpc and transferred to pseudo-pregnant females. At 11.5 dpc we observed a non-significant trend for embryonal and placental weight reduction. In embryos, the expression from the three Igf2 promoters, P0, P2, and P3, was up-regulated. In placentas, the expression level of the Igf2 promoters was not changed, except for P3, where we measured a reduction of 0.8 ± 0.14 (p = 0.05). H19 expression remained unchanged in both embryos and placentas. Our results indicate that the growth stimulating effect of ethanol observed during preimplantation is not retained through the middle of gestation, while the altered expression of Igf2 persists. This suggests that embryonal and placental growth are uncoupled from Igf2 expression following ethanol treatment, or compensatory mechanisms exist. The different response to ethanol exposure in embryos and placentas also suggests that the mechanisms of alcohol action are tissue specific.

Original languageEnglish
Pages (from-to)1661-1667
Number of pages7
JournalComptes Rendus de L'Academie Bulgare des Sciences
Volume72
Issue number12
DOIs
Publication statusPublished - 1 Jan 2019

Keywords / Materials (for Non-textual outputs)

  • Blastocyst
  • Embryo
  • Ethanol
  • Fetal alcohol syndrome
  • H19
  • Igf2

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