Projects per year
Abstract
Most long non-coding RNAs (lncRNAs) encoded by eukaryotic genomes remain uncharacterized. Here we focus on a set of intergenic lncRNAs in fission yeast. Deleting one of these lncRNAs exhibited a clear phenotype: drug sensitivity. Detailed analyses of the affected locus revealed that transcription of the nc-tgp1 lncRNA regulates drug tolerance by repressing the adjacent phosphate-responsive permease gene transporter for glycerophosphodiester 1 (tgp1(+)). We demonstrate that the act of transcribing nc-tgp1 over the tgp1(+) promoter increases nucleosome density, prevents transcription factor access and thus represses tgp1(+) without the need for RNA interference or heterochromatin components. We therefore conclude that tgp1(+) is regulated by transcriptional interference. Accordingly, decreased nc-tgp1 transcription permits tgp1(+) expression upon phosphate starvation. Furthermore, nc-tgp1 loss induces tgp1(+) even in repressive conditions. Notably, drug sensitivity results directly from tgp1(+) expression in the absence of the nc-tgp1 RNA. Thus, transcription of an lncRNA governs drug tolerance in fission yeast.
Original language | English |
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Article number | 5576 |
Journal | Nature Communications |
Volume | 5 |
DOIs | |
Publication status | Published - 2014 |
Fingerprint Dive into the research topics of 'Long non-coding RNA-mediated transcriptional interference of a permease gene confers drug tolerance in fission yeast'. Together they form a unique fingerprint.
Projects
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How centromeres are specified: the interplay between hetrochromatin, CENP-A chromatin, and kinetochore assembly
1/04/12 → 31/03/22
Project: Research
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Linking RNA interference, silent chromatin formation and kinetochore assembly.
1/04/07 → 31/03/13
Project: Research
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Chromsome segregation: centrometric chromatin, associated proteins and kinetichore assembly.
1/04/02 → 30/06/12
Project: Research
Profiles
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Robin Allshire
- School of Biological Sciences - Professor of Chromosome Biology
- SynthSys
Person: Academic: Research Active