Long-term ovarian function in sheep after ovariectomy and transplantation of autografts stored at -196 C

D T Baird, R Webb, B K Campbell, L M Harkness, R G Gosden

Research output: Contribution to journalArticlepeer-review


We have previously demonstrated that ovarian function and fertility can be preserved in sheep after castration by autotransplantation of cryopreserved strips of ovarian cortex. In the current experiments we have investigated the long term survival of such grafts by detailed measurements of ovarian function for a period of nearly 2 yr after autotransplantation. After ovariectomy and transplantation of frozen/thawed grafts, the concentrations of FSH and LH rose to castrate levels for about 14 weeks before falling gradually to reach near-normal levels at about 60 weeks. In the breeding season from October 1994 to March 1995, all ewes had 5-10 estrous cycles that were similar in length to those in the 4 control ewes. Luteal function as indicated by the progesterone concentration was identical before and 11 months after transplantation. In contrast, the basal concentrations of FSH and LH were persistently raised throughout the luteal phase, but showed a normal decline during the follicular phase. The concentration of inhibin A in ovarian venous plasma measured at the end of the experiment 22 months after transplantation was significantly lower than that in control ewes (mean +/- SE, 409 +/- 118 vs. 1914 +/- 555 pg/ml; P
Original languageEnglish
Pages (from-to)462-471
Number of pages10
Issue number1
Publication statusPublished - Jan 1999


  • Animals
  • Cryopreservation
  • Estrus
  • Female
  • Follicle Stimulating Hormone
  • Luteinizing Hormone
  • Mice
  • Mice, SCID
  • Ovariectomy
  • Ovary
  • Progesterone
  • Sheep
  • Transplantation, Autologous


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