Loss of heterozygosity occurs at the D11S29 locus on chromosome 11q23 in invasive cervical carcinoma

P B Bethwaite, J Koreth, C S Herrington, J O McGee

Research output: Contribution to journalArticlepeer-review

Abstract

Allelotypic detection of loss of heterozygosity (LOH) has been used to identify putative tumour-suppressor genes. Loci on human chromosome 11q23 are frequently altered in malignant disease, and LOH has been reported at an anonymous D11S29 locus at 11q23 in a proportion of breast and ovarian cancers and malignant melanomas. Previous studies have reported a high frequency of LOH in cervical carcinoma mapping to 11q23. Using polymerase chain reaction techniques employing probes for a recently described polymorphic dinucleotide microsatellite within this locus, we have searched for LOH in 69 cases of invasive cervical carcinoma. Genomic material was microdissected from sections cut from archival paraffin-embedded material, using the patients' constitutional genotype as a control Sixty-two (90%) of the cases were informative, and LOH occurred in 25/62 (40%) of tumours. Loss of an arm or single chromosome 11 is a well-recognised event in cervical carcinoma, and by employing other microsatellite polymorphisms mapping to 11q13 and 11p11-p12 we excluded those cases with widespread allelic loss. By doing so, LOH at D11S29 was found in 16/53 (30%) of tumours. The findings suggest a putative tumour-suppressor gene on 11q involved in cervical carcinogenesis.

Original languageEnglish
Pages (from-to)814-8
Number of pages5
JournalBritish Journal of Cancer
Volume71
Issue number4
Publication statusPublished - Apr 1995

Keywords

  • Adenocarcinoma
  • Adult
  • Age Factors
  • Base Sequence
  • Carcinoma, Squamous Cell
  • Chromosome Deletion
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11
  • DNA Primers
  • DNA, Neoplasm
  • DNA, Satellite
  • Female
  • Genetic Markers
  • Humans
  • Molecular Sequence Data
  • Neoplasm Invasiveness
  • Papillomaviridae
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • Retrospective Studies
  • Uterine Cervical Neoplasms

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