Projects per year
Abstract / Description of output
Retroelement silencing factor 1 (RESF1) interacts with the key regulators of mouse embryonic stem cells (ESCs) OCT4 and NANOG, and its absence results in sterility of mice. However, the function of RESF1 in ESCs and germ line specification is poorly understood. In this study, we used Resf1 knockout cell lines to determine the requirements of RESF1 for ESCs self-renewal and for in vitro specification of ESCs into primordial germ cell-like cells(PGCLCs). We found that deletion of Resf1 in ESCs cultured in serum and LIF reduces self renewal potential whereas episomal expression of RESF1 has a modest positive effect on ESC self-renewal. In addition, RESF1 is not required for the capacity of NANOG and its downstream target ESRRB to drive self-renewal in the absence of LIF. However, Resf1deletion reduces efficiency of PGCLC differentiation in vitro. These results identify Resf1 as a novel player in the regulation of pluripotent stem cells and germ cell specification.
Original language | English |
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Article number | e202101190 |
Number of pages | 11 |
Journal | Life Science Alliance |
Volume | 4 |
Issue number | 12 |
Early online date | 5 Oct 2021 |
DOIs | |
Publication status | E-pub ahead of print - 5 Oct 2021 |
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Dive into the research topics of 'Loss of Resf1 reduces the efficiency of embryonic stem cell self-renewal and germline entry'. Together they form a unique fingerprint.Projects
- 1 Finished
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Transcription factor control of dynamic transitions within and beyond pluripotency
1/09/19 → 31/08/24
Project: Research
Equipment
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Institute for Regeneration and Repair Flow Cytometry Facility
Shonna Johnston (Manager), Fiona Rossi (Manager), Claire Cryer (Other) & Ailsa Laird (Other)
Institute of Regeneration and RepairFacility/equipment: Facility