Lymphatic endothelial cells are essential components of the subcapsular sinus macrophage niche

Isabelle Mondor; Myriam Baratin; Marine Lagueyrie; Lisa Saro; Sandrine Henri; Rebecca Gentek; Delphine Suerinck; Wolfgang Kastenmuller; Jean X Jiang; Marc Bajénoff

doi:10.1016/j.immuni.2019.04.002

Lymphatic endothelial cells are essential components of the subcapsular sinus macrophage niche

Isabelle Mondor, Myriam Baratin, Marine Lagueyrie, Lisa Saro, Sandrine Henri, Rebecca Gentek, Delphine Suerinck, Wolfgang Kastenmuller, Jean X Jiang, Marc Bajénoff

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

In lymph nodes, subcapsular sinus macrophages (SSMs) form an immunological barrier that monitors lymph drained from peripheral tissues. Upon infection, SSMs activate B and natural killer T (NKT) cells while secreting inflammatory mediators. Here, we investigated the mechanisms regulating development and homeostasis of SSMs. Embryonic SSMs originated from yolk sac hematopoiesis and were replaced by a postnatal wave of bone marrow (BM)-derived monocytes that proliferated to establish the adult SSM network. The SSM network self-maintained by proliferation with minimal BM contribution. Upon pathogen-induced transient deletion, BM-derived cells contributed to restoring the SSM network. Lymphatic endothelial cells (LECs) were the main source of CSF-1 within the lymph node and conditional deletion of Csf1 in adult LECs decreased the network of SSMs and medullary sinus macrophages (MSMs). Thus, SSMs have a dual hematopoietic origin, and LECs are essential to the niche supporting these macrophages.

Original language	English
Pages (from-to)	1453-1466.e4
Number of pages	18
Journal	Immunity
Volume	50
Issue number	6
Early online date	30 Apr 2019
DOIs	https://doi.org/10.1016/j.immuni.2019.04.002
Publication status	Published - 18 Jun 2019

Keywords / Materials (for Non-textual outputs)

Animals
Biomarkers
Cell Communication
Cell Differentiation
Endothelial Cells/metabolism
Gene Expression
Genes, Reporter
Hematopoiesis/genetics
Homeostasis
Lymph Nodes/cytology
Lymphatic Vessels
Macrophage Colony-Stimulating Factor/metabolism
Macrophages/cytology
Mice
Monocytes/cytology
Yolk Sac

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10.1016/j.immuni.2019.04.002

Rebecca Gentek
- Deanery of Clinical Sciences - Chancellors Research Fellow (PI)
- Centre for Inflammation Research
Person: Academic: Research Active

Cite this

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title = "Lymphatic endothelial cells are essential components of the subcapsular sinus macrophage niche",

abstract = "In lymph nodes, subcapsular sinus macrophages (SSMs) form an immunological barrier that monitors lymph drained from peripheral tissues. Upon infection, SSMs activate B and natural killer T (NKT) cells while secreting inflammatory mediators. Here, we investigated the mechanisms regulating development and homeostasis of SSMs. Embryonic SSMs originated from yolk sac hematopoiesis and were replaced by a postnatal wave of bone marrow (BM)-derived monocytes that proliferated to establish the adult SSM network. The SSM network self-maintained by proliferation with minimal BM contribution. Upon pathogen-induced transient deletion, BM-derived cells contributed to restoring the SSM network. Lymphatic endothelial cells (LECs) were the main source of CSF-1 within the lymph node and conditional deletion of Csf1 in adult LECs decreased the network of SSMs and medullary sinus macrophages (MSMs). Thus, SSMs have a dual hematopoietic origin, and LECs are essential to the niche supporting these macrophages.",

keywords = "Animals, Biomarkers, Cell Communication, Cell Differentiation, Endothelial Cells/metabolism, Gene Expression, Genes, Reporter, Hematopoiesis/genetics, Homeostasis, Lymph Nodes/cytology, Lymphatic Vessels, Macrophage Colony-Stimulating Factor/metabolism, Macrophages/cytology, Mice, Monocytes/cytology, Yolk Sac",

author = "Isabelle Mondor and Myriam Baratin and Marine Lagueyrie and Lisa Saro and Sandrine Henri and Rebecca Gentek and Delphine Suerinck and Wolfgang Kastenmuller and Jiang, {Jean X} and Marc Baj{\'e}noff",

year = "2019",

month = jun,

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doi = "10.1016/j.immuni.2019.04.002",

language = "English",

volume = "50",

pages = "1453--1466.e4",

journal = "Immunity",

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T1 - Lymphatic endothelial cells are essential components of the subcapsular sinus macrophage niche

AU - Mondor, Isabelle

AU - Baratin, Myriam

AU - Lagueyrie, Marine

AU - Saro, Lisa

AU - Henri, Sandrine

AU - Gentek, Rebecca

AU - Suerinck, Delphine

AU - Kastenmuller, Wolfgang

AU - Jiang, Jean X

AU - Bajénoff, Marc

PY - 2019/6/18

Y1 - 2019/6/18

N2 - In lymph nodes, subcapsular sinus macrophages (SSMs) form an immunological barrier that monitors lymph drained from peripheral tissues. Upon infection, SSMs activate B and natural killer T (NKT) cells while secreting inflammatory mediators. Here, we investigated the mechanisms regulating development and homeostasis of SSMs. Embryonic SSMs originated from yolk sac hematopoiesis and were replaced by a postnatal wave of bone marrow (BM)-derived monocytes that proliferated to establish the adult SSM network. The SSM network self-maintained by proliferation with minimal BM contribution. Upon pathogen-induced transient deletion, BM-derived cells contributed to restoring the SSM network. Lymphatic endothelial cells (LECs) were the main source of CSF-1 within the lymph node and conditional deletion of Csf1 in adult LECs decreased the network of SSMs and medullary sinus macrophages (MSMs). Thus, SSMs have a dual hematopoietic origin, and LECs are essential to the niche supporting these macrophages.

AB - In lymph nodes, subcapsular sinus macrophages (SSMs) form an immunological barrier that monitors lymph drained from peripheral tissues. Upon infection, SSMs activate B and natural killer T (NKT) cells while secreting inflammatory mediators. Here, we investigated the mechanisms regulating development and homeostasis of SSMs. Embryonic SSMs originated from yolk sac hematopoiesis and were replaced by a postnatal wave of bone marrow (BM)-derived monocytes that proliferated to establish the adult SSM network. The SSM network self-maintained by proliferation with minimal BM contribution. Upon pathogen-induced transient deletion, BM-derived cells contributed to restoring the SSM network. Lymphatic endothelial cells (LECs) were the main source of CSF-1 within the lymph node and conditional deletion of Csf1 in adult LECs decreased the network of SSMs and medullary sinus macrophages (MSMs). Thus, SSMs have a dual hematopoietic origin, and LECs are essential to the niche supporting these macrophages.

KW - Animals

KW - Biomarkers

KW - Cell Communication

KW - Cell Differentiation

KW - Endothelial Cells/metabolism

KW - Gene Expression

KW - Genes, Reporter

KW - Hematopoiesis/genetics

KW - Homeostasis

KW - Lymph Nodes/cytology

KW - Lymphatic Vessels

KW - Macrophage Colony-Stimulating Factor/metabolism

KW - Macrophages/cytology

KW - Mice

KW - Monocytes/cytology

KW - Yolk Sac

U2 - 10.1016/j.immuni.2019.04.002

DO - 10.1016/j.immuni.2019.04.002

M3 - Article

C2 - 31053503

SN - 1074-7613

VL - 50

SP - 1453-1466.e4

JO - Immunity

JF - Immunity

IS - 6

ER -

Lymphatic endothelial cells are essential components of the subcapsular sinus macrophage niche

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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Rebecca Gentek

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