Lymphatic endothelial cells are essential components of the subcapsular sinus macrophage niche

Isabelle Mondor, Myriam Baratin, Marine Lagueyrie, Lisa Saro, Sandrine Henri, Rebecca Gentek, Delphine Suerinck, Wolfgang Kastenmuller, Jean X Jiang, Marc Bajénoff

Research output: Contribution to journalArticlepeer-review

Abstract

In lymph nodes, subcapsular sinus macrophages (SSMs) form an immunological barrier that monitors lymph drained from peripheral tissues. Upon infection, SSMs activate B and natural killer T (NKT) cells while secreting inflammatory mediators. Here, we investigated the mechanisms regulating development and homeostasis of SSMs. Embryonic SSMs originated from yolk sac hematopoiesis and were replaced by a postnatal wave of bone marrow (BM)-derived monocytes that proliferated to establish the adult SSM network. The SSM network self-maintained by proliferation with minimal BM contribution. Upon pathogen-induced transient deletion, BM-derived cells contributed to restoring the SSM network. Lymphatic endothelial cells (LECs) were the main source of CSF-1 within the lymph node and conditional deletion of Csf1 in adult LECs decreased the network of SSMs and medullary sinus macrophages (MSMs). Thus, SSMs have a dual hematopoietic origin, and LECs are essential to the niche supporting these macrophages.

Original languageEnglish
Pages (from-to)1453-1466.e4
Number of pages18
JournalImmunity
Volume50
Issue number6
Early online date30 Apr 2019
DOIs
Publication statusPublished - 18 Jun 2019

Keywords

  • Animals
  • Biomarkers
  • Cell Communication
  • Cell Differentiation
  • Endothelial Cells/metabolism
  • Gene Expression
  • Genes, Reporter
  • Hematopoiesis/genetics
  • Homeostasis
  • Lymph Nodes/cytology
  • Lymphatic Vessels
  • Macrophage Colony-Stimulating Factor/metabolism
  • Macrophages/cytology
  • Mice
  • Monocytes/cytology
  • Yolk Sac

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