Lymphocytes modulate peritoneal leukocyte recruitment in peritonitis

T. Kipari, S. Watson, K. Houlberg, S. Lepage, J. Hughes, Jean-Francois Cailhier

Research output: Contribution to journalArticlepeer-review

Abstract

To investigate the modulating role of lymphocytes in leukocyte recruitment in a murine model of peritonitis.

RAG-1 knockout (KO) mice, NUDE mice and mu MT KO mice were compared to their wild-type controls.

Mice were administered with 1 ml of Brewer's thioglycollate (BTG) and terminal peritoneal lavages were performed at 8, 24, 72 and 120 h after treatment.

Leukocyte numbers recruited at the different time points following a BTG administration were determined. Chemokine and cytokine levels were assessed by either ELISAs or cytometric bead array.

RAG-1 KO mice (absent B and T cells) exhibited increased early neutrophil infiltration and blunted late monocyte/macrophage infiltration. NUDE mice (absent T cells) exhibited both increased neutrophil and monocyte/macrophage infiltration. In contrast, mu MT KO mice (absent B cells) demonstrated reduced influx of both neutrophils and monocyte/macrophages. Chemokine analysis revealed various differences in important chemokines.

These data suggest that T cells act to suppress leukocyte recruitment while B cells promote leukocyte recruitment.

Original languageEnglish
Pages (from-to)553-560
Number of pages8
JournalInflammation Research
Volume58
Issue number9
DOIs
Publication statusPublished - Sep 2009

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