Macrophage chemotaxis to apoptotic Burkitt's lymphoma cells in vitro: role of CD14 and CD36

Lucy A Truman; Carol Anne Ogden; Sarah E M Howie; Christopher D Gregory

doi:10.1016/j.imbio.2004.02.001

Macrophage chemotaxis to apoptotic Burkitt's lymphoma cells in vitro: role of CD14 and CD36

Lucy A Truman, Carol Anne Ogden, Sarah E M Howie, Christopher D Gregory

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

In Burkitt's lymphoma (BL), apoptosis occurs at high frequency alongside uncontrolled proliferation. Macrophages infiltrate these tumours in large numbers and engage in the phagocytic clearance of apoptotic cells in situ. Here we tested the hypothesis that apoptosis of BL cells may provide a mechanism for recruitment of macrophages to these tumours. We show that monocytes and macrophages, but not neutrophils, preferentially migrated to apoptotic BL cells in vitro. Transfection of BL cells with the anti-apoptotic gene bcl-2 both prevented apoptosis and abolished macrophage chemotaxis. Macrophage migration to BL populations correlated well with the number of apoptotic BL cells present (the Pearson correlation r = 0.81, p

Original language	English
Pages (from-to)	21-30
Number of pages	10
Journal	Immunobiology
Volume	209
Issue number	1-2
DOIs	https://doi.org/10.1016/j.imbio.2004.02.001
Publication status	Published - 2004

Keywords / Materials (for Non-textual outputs)

Animals
Antigens, CD14
Antigens, CD36
Apoptosis
Burkitt Lymphoma
Cells, Cultured
Chemotaxis
Flow Cytometry
Humans
Macrophages
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Tumor Cells, Cultured
Up-Regulation

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10.1016/j.imbio.2004.02.001

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@article{164a896979ea4a6fa5b6b45db329f482,

title = "Macrophage chemotaxis to apoptotic Burkitt's lymphoma cells in vitro: role of CD14 and CD36",

abstract = "In Burkitt's lymphoma (BL), apoptosis occurs at high frequency alongside uncontrolled proliferation. Macrophages infiltrate these tumours in large numbers and engage in the phagocytic clearance of apoptotic cells in situ. Here we tested the hypothesis that apoptosis of BL cells may provide a mechanism for recruitment of macrophages to these tumours. We show that monocytes and macrophages, but not neutrophils, preferentially migrated to apoptotic BL cells in vitro. Transfection of BL cells with the anti-apoptotic gene bcl-2 both prevented apoptosis and abolished macrophage chemotaxis. Macrophage migration to BL populations correlated well with the number of apoptotic BL cells present (the Pearson correlation r = 0.81, p",

keywords = "Animals, Antigens, CD14, Antigens, CD36, Apoptosis, Burkitt Lymphoma, Cells, Cultured, Chemotaxis, Flow Cytometry, Humans, Macrophages, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Tumor Cells, Cultured, Up-Regulation",

author = "Truman, {Lucy A} and Ogden, {Carol Anne} and Howie, {Sarah E M} and Gregory, {Christopher D}",

year = "2004",

doi = "10.1016/j.imbio.2004.02.001",

language = "English",

volume = "209",

pages = "21--30",

journal = "Immunobiology",

issn = "0171-2985",

publisher = "Urban und Fischer Verlag GmbH und Co. KG",

number = "1-2",

}

TY - JOUR

T1 - Macrophage chemotaxis to apoptotic Burkitt's lymphoma cells in vitro

T2 - role of CD14 and CD36

AU - Truman, Lucy A

AU - Ogden, Carol Anne

AU - Howie, Sarah E M

AU - Gregory, Christopher D

PY - 2004

Y1 - 2004

N2 - In Burkitt's lymphoma (BL), apoptosis occurs at high frequency alongside uncontrolled proliferation. Macrophages infiltrate these tumours in large numbers and engage in the phagocytic clearance of apoptotic cells in situ. Here we tested the hypothesis that apoptosis of BL cells may provide a mechanism for recruitment of macrophages to these tumours. We show that monocytes and macrophages, but not neutrophils, preferentially migrated to apoptotic BL cells in vitro. Transfection of BL cells with the anti-apoptotic gene bcl-2 both prevented apoptosis and abolished macrophage chemotaxis. Macrophage migration to BL populations correlated well with the number of apoptotic BL cells present (the Pearson correlation r = 0.81, p

AB - In Burkitt's lymphoma (BL), apoptosis occurs at high frequency alongside uncontrolled proliferation. Macrophages infiltrate these tumours in large numbers and engage in the phagocytic clearance of apoptotic cells in situ. Here we tested the hypothesis that apoptosis of BL cells may provide a mechanism for recruitment of macrophages to these tumours. We show that monocytes and macrophages, but not neutrophils, preferentially migrated to apoptotic BL cells in vitro. Transfection of BL cells with the anti-apoptotic gene bcl-2 both prevented apoptosis and abolished macrophage chemotaxis. Macrophage migration to BL populations correlated well with the number of apoptotic BL cells present (the Pearson correlation r = 0.81, p

KW - Animals

KW - Antigens, CD14

KW - Antigens, CD36

KW - Apoptosis

KW - Burkitt Lymphoma

KW - Cells, Cultured

KW - Chemotaxis

KW - Flow Cytometry

KW - Humans

KW - Macrophages

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Inbred C57BL

KW - Tumor Cells, Cultured

KW - Up-Regulation

U2 - 10.1016/j.imbio.2004.02.001

DO - 10.1016/j.imbio.2004.02.001

M3 - Article

C2 - 15481137

SN - 0171-2985

VL - 209

SP - 21

EP - 30

JO - Immunobiology

JF - Immunobiology

IS - 1-2

ER -

Macrophage chemotaxis to apoptotic Burkitt's lymphoma cells in vitro: role of CD14 and CD36

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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