Macrophages from Susceptible and Resistant Chicken Lines have Different Transcriptomes following Marek’s Disease Virus Infection

Pankaj Chakraborty; Richard Kuo; Lonneke Vervelde; Bernadette Dutia; Peter Kaiser; Jacqueline Smith

doi:10.3390/genes10020074

Macrophages from Susceptible and Resistant Chicken Lines have Different Transcriptomes following Marek’s Disease Virus Infection

Pankaj Chakraborty
, Richard Kuo
, Lonneke Vervelde
, Bernadette Dutia
, Peter Kaiser
, Jacqueline Smith

Research output: Contribution to journal › Article › peer-review

Abstract

Despite successful control by vaccination, Marek’s disease (MD) has continued evolving to greater virulence over recent years. To control MD, selection and breeding of MD-resistant chickens might be a suitable option. MHC-congenic inbred chicken lines, 61 and 72, are highly resistant and susceptible to MD, respectively, but the cellular and genetic basis for these phenotypes is unknown. MD virus (MDV) infects macrophages, B-cells and activated T-cells in vivo. This study investigates the cellular basis of resistance to MD in vitro with the hypothesis that resistance is determined by cells active during the innate immune response. Chicken bone marrow-derived macrophages from lines 61 and 72 were infected with MDV in vitro. Flow cytometry showed that a higher percentage of macrophages were infected in line 72 than in line 61. A transcriptomic study followed by in silico functional analysis of differentially expressed genes was then carried out between the two lines pre- and post-infection. Analysis supports the hypothesis that macrophages from susceptible and resistant chicken lines display a marked difference in their transcriptome following MDV infection. Resistance to infection, differential activation of biological pathways and suppression of oncogenic potential are among host defense strategies identified in macrophages from resistant chickens.

Original language	English
Journal	Genes
DOIs	https://doi.org/10.3390/genes10020074
Publication status	Published - 22 Jan 2019

Keywords / Materials (for Non-textual outputs)

chickens
Marek's disease virus
disease resistance
macrophages
RNA - sequence

Access to Document

10.3390/genes10020074

Macrophages from Susceptible and Resistant
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Final published version, 1.1 MBLicence: Creative Commons: Attribution (CC-BY)

ISP4 Pathogenesis and resistance in viral diseases of livestock
Dutia, B. (Principal Investigator), Archibald, A. (Co-investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Collie, D. (Co-investigator), Digard, P. (Co-investigator), Freeman, T. (Co-investigator), Glass, E. (Co-investigator), Grey, F. (Co-investigator), Hocking, P. (Co-investigator), Houston, R. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Nash, A. (Co-investigator), Sang, H. (Co-investigator), Sharp, C. (Co-investigator), Watson, M. (Co-investigator) & Whitelaw, B. (Co-investigator)
Biotechnology and Biological Sciences Research Council
1/04/12 → 31/03/17
Project: Research

Jacqueline Smith
- Jacqueline.smithroslin.ed.acuk
- Royal (Dick) School of Veterinary Studies - Senior Research Fellow
Person: Academic: Research Active (Research Assistant)

Cite this

@article{7d68e06fbf7f4e78beba68454a09663a,

title = "Macrophages from Susceptible and Resistant Chicken Lines have Different Transcriptomes following Marek{\textquoteright}s Disease Virus Infection",

abstract = " Despite successful control by vaccination, Marek{\textquoteright}s disease (MD) has continued evolving to greater virulence over recent years. To control MD, selection and breeding of MD-resistant chickens might be a suitable option. MHC-congenic inbred chicken lines, 61 and 72, are highly resistant and susceptible to MD, respectively, but the cellular and genetic basis for these phenotypes is unknown. MD virus (MDV) infects macrophages, B-cells and activated T-cells in vivo. This study investigates the cellular basis of resistance to MD in vitro with the hypothesis that resistance is determined by cells active during the innate immune response. Chicken bone marrow-derived macrophages from lines 61 and 72 were infected with MDV in vitro. Flow cytometry showed that a higher percentage of macrophages were infected in line 72 than in line 61. A transcriptomic study followed by in silico functional analysis of differentially expressed genes was then carried out between the two lines pre- and post-infection. Analysis supports the hypothesis that macrophages from susceptible and resistant chicken lines display a marked difference in their transcriptome following MDV infection. Resistance to infection, differential activation of biological pathways and suppression of oncogenic potential are among host defense strategies identified in macrophages from resistant chickens. ",

keywords = "chickens, Marek's disease virus, disease resistance, macrophages, RNA - sequence",

author = "Pankaj Chakraborty and Richard Kuo and Lonneke Vervelde and Bernadette Dutia and Peter Kaiser and Jacqueline Smith",

year = "2019",

month = jan,

day = "22",

doi = "10.3390/genes10020074",

language = "English",

journal = "Genes",

issn = "2073-4425",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

}

TY - JOUR

T1 - Macrophages from Susceptible and Resistant Chicken Lines have Different Transcriptomes following Marek’s Disease Virus Infection

AU - Chakraborty , Pankaj

AU - Kuo, Richard

AU - Vervelde, Lonneke

AU - Dutia, Bernadette

AU - Kaiser, Peter

AU - Smith, Jacqueline

PY - 2019/1/22

Y1 - 2019/1/22

N2 - Despite successful control by vaccination, Marek’s disease (MD) has continued evolving to greater virulence over recent years. To control MD, selection and breeding of MD-resistant chickens might be a suitable option. MHC-congenic inbred chicken lines, 61 and 72, are highly resistant and susceptible to MD, respectively, but the cellular and genetic basis for these phenotypes is unknown. MD virus (MDV) infects macrophages, B-cells and activated T-cells in vivo. This study investigates the cellular basis of resistance to MD in vitro with the hypothesis that resistance is determined by cells active during the innate immune response. Chicken bone marrow-derived macrophages from lines 61 and 72 were infected with MDV in vitro. Flow cytometry showed that a higher percentage of macrophages were infected in line 72 than in line 61. A transcriptomic study followed by in silico functional analysis of differentially expressed genes was then carried out between the two lines pre- and post-infection. Analysis supports the hypothesis that macrophages from susceptible and resistant chicken lines display a marked difference in their transcriptome following MDV infection. Resistance to infection, differential activation of biological pathways and suppression of oncogenic potential are among host defense strategies identified in macrophages from resistant chickens.

AB - Despite successful control by vaccination, Marek’s disease (MD) has continued evolving to greater virulence over recent years. To control MD, selection and breeding of MD-resistant chickens might be a suitable option. MHC-congenic inbred chicken lines, 61 and 72, are highly resistant and susceptible to MD, respectively, but the cellular and genetic basis for these phenotypes is unknown. MD virus (MDV) infects macrophages, B-cells and activated T-cells in vivo. This study investigates the cellular basis of resistance to MD in vitro with the hypothesis that resistance is determined by cells active during the innate immune response. Chicken bone marrow-derived macrophages from lines 61 and 72 were infected with MDV in vitro. Flow cytometry showed that a higher percentage of macrophages were infected in line 72 than in line 61. A transcriptomic study followed by in silico functional analysis of differentially expressed genes was then carried out between the two lines pre- and post-infection. Analysis supports the hypothesis that macrophages from susceptible and resistant chicken lines display a marked difference in their transcriptome following MDV infection. Resistance to infection, differential activation of biological pathways and suppression of oncogenic potential are among host defense strategies identified in macrophages from resistant chickens.

KW - chickens

KW - Marek's disease virus

KW - disease resistance

KW - macrophages

KW - RNA - sequence

U2 - 10.3390/genes10020074

DO - 10.3390/genes10020074

M3 - Article

SN - 2073-4425

JO - Genes

JF - Genes

ER -

Macrophages from Susceptible and Resistant Chicken Lines have Different Transcriptomes following Marek’s Disease Virus Infection

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Projects

ISP4 Pathogenesis and resistance in viral diseases of livestock

Profiles

Jacqueline Smith

Cite this