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Abstract / Description of output
The origins and functions of kidney macrophages in the adult have been explored, but their roles during development remain largely unknown. Here we characterise macrophage arrival, localisation, heterogeneity, and functions during kidney organogenesis. Using genetic approaches to ablate macrophages, we identify a role for macrophages in nephron progenitor cell clearance as mouse kidney development begins. Throughout renal organogenesis, most kidney macrophages are perivascular and express F4/80 and CD206. These macrophages are enriched for mRNAs linked to developmental processes, such as blood vessel morphogenesis. Using antibody-mediated macrophage-depletion, we show macrophages support vascular anastomoses in cultured kidney explants. We also characterise a subpopulation of galectin-3+ (Gal3+) myeloid cells within the developing kidney. Our findings may stimulate research into macrophage-based therapies for renal developmental abnormalities and have implications for the generation of bioengineered kidney tissues.
Original language | English |
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Article number | e43271 |
Journal | eLIFE |
Volume | 8 |
Early online date | 13 Feb 2019 |
DOIs | |
Publication status | Published - 13 Feb 2019 |
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Dive into the research topics of 'Macrophages restrict the nephrogenic field and promote endothelial connections during kidney development'. Together they form a unique fingerprint.Projects
- 1 Finished
Profiles
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Jamie Davies, FRSM
- Deanery of Biomedical Sciences - Personal Chair of Experimental Anatomy
- Centre for Discovery Brain Sciences
- Centre for Engineering Biology
Person: Academic: Research Active