Madin-Darby bovine kidney (MDBK) cells are a suitable cell line for the propagation and study of the bovine poxvirus lumpy skin disease virus

Petra Fay, Charlotte Cook, Najith Wijesiriwardana, Gessica Tore, Loic Comtet, Alix Carpentier, Barbara Shih, Graham Freimanis, Ismar R Haga, Philippa M Beard

Research output: Contribution to journalArticlepeer-review

Abstract

Lumpy skin disease virus (LSDV) is a poxvirus that causes systemic disease in cattle, resulting in substantial economic loss to affected communities. LSDV is a rapidly emerging pathogen of growing global concern that recently spread from Africa and the Middle East into Europe and Asia, impacting the cattle population in these regions. An increase in research efforts into LSDV is required to address key knowledge gaps, however this is hampered by lack of suitable cell lines on which to propagate and study the virus. In this work we describe the replication and spread of LSDV on Madin-Darby bovine kidney (MDBK) cells, and the formation of foci-type poxvirus plaques by LSDV on MDBK cells. Methods utilising MDBK cells to quantify neutralising antibodies to LSDV, and to purify LSDV genomic DNA suitable for short read sequencing are described. These research methods broaden the tools available for LSDV researchers and will facilitate the gathering of evidence to underpin the development of LSD control and prevention programmes.

Original languageEnglish
Pages (from-to)113943
JournalJournal of Virological Methods
Early online date22 Jul 2020
DOIs
Publication statusE-pub ahead of print - 22 Jul 2020

Keywords

  • Lumpy skin disease virus
  • Poxvirus
  • Capripoxvirus
  • Neutralization
  • Viral genome sequencing

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