Making immortalized cell lines from embryonic mouse kidney

Guanping Tai; Peter Hohenstein; Jamie A Davies

doi:10.1007/978-1-61779-851-1_15

Making immortalized cell lines from embryonic mouse kidney

Guanping Tai, Peter Hohenstein, Jamie A Davies

Research output: Contribution to journal › Article › peer-review

Abstract

Immortalized cell lines derived from embryonic mouse kidneys are useful tools for exploring signaling pathways, morphogenetic mechanisms, and gene function in renal development: they also provide a means for efficient first-round screening of panels of small molecules intended to combat renal pathologies such as the development of cysts, and such cell line-based screening can allow a valuable reduction in the numbers of animals needed for a given line of research. This chapter presents a simple method for generating cell lines from the "Immortomouse," which carries a temperature-sensitive SV40 antigen, under the control of an interferon-regulated promoter.

Original language	English
Pages (from-to)	165-71
Number of pages	7
Journal	Methods in Molecular Biology
Volume	886
DOIs	https://doi.org/10.1007/978-1-61779-851-1_15
Publication status	Published - 2012

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10.1007/978-1-61779-851-1_15

Making immortalized cell lines from embryonic mouse kidney
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Reducing mouse no complex genetic exper
Hohenstein, P.
UK central government bodies/local authorities, health and hospital authorities
1/10/12 → 9/07/14
Project: Research

Cite this

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AB - Immortalized cell lines derived from embryonic mouse kidneys are useful tools for exploring signaling pathways, morphogenetic mechanisms, and gene function in renal development: they also provide a means for efficient first-round screening of panels of small molecules intended to combat renal pathologies such as the development of cysts, and such cell line-based screening can allow a valuable reduction in the numbers of animals needed for a given line of research. This chapter presents a simple method for generating cell lines from the "Immortomouse," which carries a temperature-sensitive SV40 antigen, under the control of an interferon-regulated promoter.

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JO - Methods in Molecular Biology

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Making immortalized cell lines from embryonic mouse kidney

Abstract

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Reducing mouse no complex genetic exper

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