MALDI Matrix Application Utilizing a Modified 3D Printer for Accessible High Resolution Mass Spectrometry Imaging

Lulu Tucker, Antonio Conde-gonzález, Diego Cobice, Gregory Hamm, Richard J. A. Goodwin, Colin J. Campbell, David James Clarke, Colin Logan Mackay

Research output: Contribution to journalArticlepeer-review

Abstract

Successful matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI) relies on the selection of the most appropriate matrix and optimization of the matrix application parameters. In order to achieve reproducible high spatial-resolution imaging data, several commercially available automated matrix application platforms have become available. How-ever, the high cost of these commercial matrix sprayers is restricting access into this emerging research field. Here, we report an automated platform for matrix deposition, employing a converted commercially-available 3D printer ($300) and other parts commonly found in an analytical chemistry lab alow-cost alternative to commercial sprayers. Using printed fluorescent rhodamine B microarrays and employing experimental design, the matrix deposition parameters were optimized to minimize surface analyte diffusion. Finally, the optimized matrix application method was applied to image 3-dimensional MCF-7 cell culture spheroid sections (ca. 500 μm diameter tissue samples) and sections of mouse brain. Using this system, we demonstrate robust and reproducible obser-vations of endogenous metabolite and steroid distributions with a high spatial resolution.
Original languageEnglish
JournalAnalytical Chemistry
Early online date4 Jun 2018
DOIs
Publication statusE-pub ahead of print - 4 Jun 2018

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