Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid

Jaakko L O Pohjoismäki, J Bradley Holmes, Stuart R Wood, Ming-Yao Yang, Takehiro Yasukawa, Aurelio Reyes, Laura J Bailey, Tricia J Cluett, Steffi Goffart, Smaranda Willcox, Rachel E Rigby, Andrew P Jackson, Johannes N Spelbrink, Jack D Griffith, Robert J Crouch, Howard T Jacobs, Ian J Holt

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorporation throughout the lagging strand.
Original languageEnglish
Pages (from-to)1144-55
Number of pages12
JournalJournal of Molecular Biology
Volume397
Issue number5
DOIs
Publication statusPublished - 2010

Keywords

  • DNA replication
  • mitochondrial DNA
  • RNA/DNA hybrid
  • electrophoresis
  • electron microscopy

Fingerprint

Dive into the research topics of 'Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid'. Together they form a unique fingerprint.

Cite this