Manipulating Pericyte Function with MicroRNAs

Milena Vitiello, Ben Cathcart, Andrea Caporali, Marco Meloni

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract / Description of output

MicroRNAs (miRNAs) are expressed in all cell types, including pericytes, and play essential roles in vascular development, homeostasis, and disease. Manipulation of pericytes with miRNA mimics and inhibitors represents an essential tool to study the role of pericytes in vascular development and regeneration and to better understand the therapeutic potential of miRNA manipulation in pericytes. Here we describe methods for manipulating pericyte function by using miRNA mimics and inhibitors. We also describe methods to assess pericyte function (proliferation and migration) after manipulation with miRNAs and explain how miRNA gene targets can be identified and validated in pericytes after manipulation with miRNA.

Original languageEnglish
Title of host publicationPericytes
Subtitle of host publicationMethods and Protocols
EditorsBruno M. Péault
PublisherHumana, New York, NY
Number of pages15
Publication statusPublished - 13 Feb 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Cloning, Molecular/methods
  • Gene Expression Regulation/genetics
  • Humans
  • MicroRNAs/genetics
  • Pericytes/metabolism
  • Transfection/methods
  • Transformation, Genetic/genetics


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