Mapping and SNP analysis of bovine candidate genes for meat and carcass quality

A. Haegeman, John Williams, A. Law, A. Van Zeveren, L. J. Peelman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The chromosomal localization of 13 bovine genes was determined using radiation hybrid (RH) mapping. The RH mapping data were in agreement with published data using either linkage, somatic cell hybrids or in situ hybridization. Mutation analysis using single-stranded conformational polymorphism, restriction fragment length polymorphism (RFLP) and sequencing revealed 13 SNPs in four different genes, namely carboxypeptidase E (CPE), uncoupling protein 2 (UCP2), single-minded (Drosophila) homologue 1 (SIM1) and methallothionein IIa (MT2A). With the exception of one mutation in CPE, all other mutations are either silent or are situated in an intron. The polymerase chain reaction RFLP was used on unrelated animals from different cattle breeds for determing allelic distribution.

Original languageEnglish
Pages (from-to)349-353
Number of pages5
JournalAnimal Genetics
Volume34
Issue number5
DOIs
Publication statusPublished - 1 Oct 2003

Keywords

  • Radiation hybrid mapping
  • RFLP
  • SNP
  • SSCP

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