Mapping quantitative trait loci regions associated with Marek's disease on chicken autosomes by means of selective DNA pooling

Ehud Lipkin, Jacqueline Smith, Morris Soller, David W Burt, Janet E Fulton

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Marek's Disease (MD), which can result in neurological damage and tumour formation, has large effects on the economy and animal welfare of the poultry industry worldwide. Previously, we mapped autosomal MD QTL regions (QTLRs) by individual genotyping of an F6 population from a full-sib advanced intercross line. We further mapped MD QTLRs on the chicken Z chromosome (GGZ) using the same F6 population, and by selective DNA pooling (SDP) of 8 elite egg production lines. Here we used SDP of the same pools used on GGZ to map autosomal MD QTLRs. Thirty-seven QTLRs were found. Seven of the QTLRs were tested by all sires from the same 8 lines, individually genotyped for QTLR markers. Five of the tested QTLRs were confirmed. Linkage disequilibrium (LD) was calculated for all QTLR markers on the same chromosome, and complex LD blocks were found. Distribution of P and LD values were used to assess the QTLR causative elements. Allele substitution effects were calculated based on both pooled SNP microarray genotypes, and individual genotypes of QTLRs markers. Substantial allele effect and contribution to the phenotypic and genotypic variation were obtained. The results explain part of the MD response, and provide targets for mitigating MD.

Original languageEnglish
Article number31896
Pages (from-to)1-13
Number of pages13
JournalScientific Reports
Volume14
Issue number1
Early online date30 Dec 2024
DOIs
Publication statusE-pub ahead of print - 30 Dec 2024

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Quantitative Trait Loci
  • Chickens/genetics
  • Marek Disease/genetics
  • Chromosome Mapping
  • Linkage Disequilibrium
  • Polymorphism, Single Nucleotide
  • Genotype
  • Alleles
  • Female
  • Male

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