Marker-assisted introgression using non-unique marker alleles I: Selection on the presence of linked marker alleles

A. M. Van Heelsum*, P. M. Visscher, C. S. Haley

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This paper investigates marker-assisted introgression of a major gene into an outbred line, where identification of the introgressed gene is incomplete because marker alleles are not unique to the base populations (the same marker allele can occur in both donor and recipient population). Those markers are used to identify the introgressed allele as well as the background genotype. The effect of using those markers, as if they were completely informative on the retention of the introgressed allele, was examined over five generations of backcrossing by using a single marker or a marker bracket for different starting frequencies of the marker alleles. Results were calculated by using both a deterministic approach, where selection is only for the desired allele, and by a stochastic approach, where selection is also on background genotype. When marker allele frequencies in donor and recipient population diverged from i and 0 (using a diallelic marker), the ability to retain the desired allele rapidly declined. Marker brackets performed notably better than single markers. If selection on background marker genotype was applied, the desired allele could be lost even more quickly than expected at random because the chance that the allele, which is common in the donor line, is present on the locus identifying the introgressed allele and is surrounded by alleles common in the recipient line on the background marker loci, will descend from the donor line (double recombination has taken place), is a lot smaller than the chance that this allele will stem from the recipient line (in which the allele occurs in low frequency). Marker brackets again performed better. Preselection against marker homozygotes (producing uninformative gametes) gave a slightly better retention of the introgressed allele.

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalAnimal Genetics
Volume28
Issue number3
Publication statusPublished - 1 Jun 1997

Keywords

  • Animal breeding
  • Marker-assisted introgression
  • Outbred populations
  • Quantitative trait loci

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