Mass spectrometric detection of candidate protein biomarkers of cancer cachexia in human urine

Richard J E Skipworth; Grant D Stewart; Mishaim Bhana; Jennifer Christie; Catharine M Sturgeon; Denis C Guttridge; Andrew D Cronshaw; Kenneth C H Fearon; James A Ross

doi:10.3892/ijo_00000577

Mass spectrometric detection of candidate protein biomarkers of cancer cachexia in human urine

Richard J E Skipworth, Grant D Stewart, Mishaim Bhana, Jennifer Christie, Catharine M Sturgeon, Denis C Guttridge, Andrew D Cronshaw, Kenneth C H Fearon, James A Ross

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Increased membrane permeability and myofibrillar protein breakdown are established features of cancer cachexia. Proteins released from cachectic muscle may be excreted in urine to act as biomarkers of the cachectic process. One-dimensional SDS polyacrylamide gel electrophoresis followed by matrix-assisted laser desorption/ionisation or liquid chromatography tandem mass spectrometry was used to compare the protein content of urine from cachectic (>10% weight loss) (n=8) and weight-stable (n=8) gastro-oesophageal cancer patients and healthy controls (n=8). Plasma creatine kinase concentration was used as a marker of gross muscle breakdown. The number of protein species identified in cachectic samples (median 42; range 28-61; total 199) was greater than that identified in weight-stable cancer (median 15; range 9-28; total 79) and control samples (median 12.5; range 5-18; total 49) (P

Original language	English
Pages (from-to)	973-82
Number of pages	10
Journal	International journal of oncology
Volume	36
Issue number	4
DOIs	https://doi.org/10.3892/ijo_00000577
Publication status	Published - Apr 2010

Keywords / Materials (for Non-textual outputs)

Adult
Aged
Aged, 80 and over
Biological Markers
Cachexia
Case-Control Studies
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Esophageal Neoplasms
Female
Humans
Male
Middle Aged
Muscle Proteins
Predictive Value of Tests
Proteinuria
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stomach Neoplasms
Tandem Mass Spectrometry
Young Adult

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10.3892/ijo_00000577

Cite this

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title = "Mass spectrometric detection of candidate protein biomarkers of cancer cachexia in human urine",

abstract = "Increased membrane permeability and myofibrillar protein breakdown are established features of cancer cachexia. Proteins released from cachectic muscle may be excreted in urine to act as biomarkers of the cachectic process. One-dimensional SDS polyacrylamide gel electrophoresis followed by matrix-assisted laser desorption/ionisation or liquid chromatography tandem mass spectrometry was used to compare the protein content of urine from cachectic (>10% weight loss) (n=8) and weight-stable (n=8) gastro-oesophageal cancer patients and healthy controls (n=8). Plasma creatine kinase concentration was used as a marker of gross muscle breakdown. The number of protein species identified in cachectic samples (median 42; range 28-61; total 199) was greater than that identified in weight-stable cancer (median 15; range 9-28; total 79) and control samples (median 12.5; range 5-18; total 49) (P",

keywords = "Adult, Aged, Aged, 80 and over, Biological Markers, Cachexia, Case-Control Studies, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Esophageal Neoplasms, Female, Humans, Male, Middle Aged, Muscle Proteins, Predictive Value of Tests, Proteinuria, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Stomach Neoplasms, Tandem Mass Spectrometry, Young Adult",

author = "Skipworth, {Richard J E} and Stewart, {Grant D} and Mishaim Bhana and Jennifer Christie and Sturgeon, {Catharine M} and Guttridge, {Denis C} and Cronshaw, {Andrew D} and Fearon, {Kenneth C H} and Ross, {James A}",

year = "2010",

month = apr,

doi = "10.3892/ijo_00000577",

language = "English",

volume = "36",

pages = "973--82",

journal = "International journal of oncology",

issn = "1019-6439",

publisher = "Spandidos Publications",

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T1 - Mass spectrometric detection of candidate protein biomarkers of cancer cachexia in human urine

AU - Skipworth, Richard J E

AU - Stewart, Grant D

AU - Bhana, Mishaim

AU - Christie, Jennifer

AU - Sturgeon, Catharine M

AU - Guttridge, Denis C

AU - Cronshaw, Andrew D

AU - Fearon, Kenneth C H

AU - Ross, James A

PY - 2010/4

Y1 - 2010/4

N2 - Increased membrane permeability and myofibrillar protein breakdown are established features of cancer cachexia. Proteins released from cachectic muscle may be excreted in urine to act as biomarkers of the cachectic process. One-dimensional SDS polyacrylamide gel electrophoresis followed by matrix-assisted laser desorption/ionisation or liquid chromatography tandem mass spectrometry was used to compare the protein content of urine from cachectic (>10% weight loss) (n=8) and weight-stable (n=8) gastro-oesophageal cancer patients and healthy controls (n=8). Plasma creatine kinase concentration was used as a marker of gross muscle breakdown. The number of protein species identified in cachectic samples (median 42; range 28-61; total 199) was greater than that identified in weight-stable cancer (median 15; range 9-28; total 79) and control samples (median 12.5; range 5-18; total 49) (P

AB - Increased membrane permeability and myofibrillar protein breakdown are established features of cancer cachexia. Proteins released from cachectic muscle may be excreted in urine to act as biomarkers of the cachectic process. One-dimensional SDS polyacrylamide gel electrophoresis followed by matrix-assisted laser desorption/ionisation or liquid chromatography tandem mass spectrometry was used to compare the protein content of urine from cachectic (>10% weight loss) (n=8) and weight-stable (n=8) gastro-oesophageal cancer patients and healthy controls (n=8). Plasma creatine kinase concentration was used as a marker of gross muscle breakdown. The number of protein species identified in cachectic samples (median 42; range 28-61; total 199) was greater than that identified in weight-stable cancer (median 15; range 9-28; total 79) and control samples (median 12.5; range 5-18; total 49) (P

KW - Adult

KW - Aged

KW - Aged, 80 and over

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KW - Cachexia

KW - Case-Control Studies

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KW - Electrophoresis, Polyacrylamide Gel

KW - Esophageal Neoplasms

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Muscle Proteins

KW - Predictive Value of Tests

KW - Proteinuria

KW - Proteomics

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Stomach Neoplasms

KW - Tandem Mass Spectrometry

KW - Young Adult

U2 - 10.3892/ijo_00000577

DO - 10.3892/ijo_00000577

M3 - Article

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SN - 1019-6439

VL - 36

SP - 973

EP - 982

JO - International journal of oncology

JF - International journal of oncology

IS - 4

ER -

Mass spectrometric detection of candidate protein biomarkers of cancer cachexia in human urine

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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