Mass spectrometric detection of candidate protein biomarkers of cancer cachexia in human urine

Richard J E Skipworth, Grant D Stewart, Mishaim Bhana, Jennifer Christie, Catharine M Sturgeon, Denis C Guttridge, Andrew D Cronshaw, Kenneth C H Fearon, James A Ross

Research output: Contribution to journalArticlepeer-review

Abstract

Increased membrane permeability and myofibrillar protein breakdown are established features of cancer cachexia. Proteins released from cachectic muscle may be excreted in urine to act as biomarkers of the cachectic process. One-dimensional SDS polyacrylamide gel electrophoresis followed by matrix-assisted laser desorption/ionisation or liquid chromatography tandem mass spectrometry was used to compare the protein content of urine from cachectic (>10% weight loss) (n=8) and weight-stable (n=8) gastro-oesophageal cancer patients and healthy controls (n=8). Plasma creatine kinase concentration was used as a marker of gross muscle breakdown. The number of protein species identified in cachectic samples (median 42; range 28-61; total 199) was greater than that identified in weight-stable cancer (median 15; range 9-28; total 79) and control samples (median 12.5; range 5-18; total 49) (P
Original languageEnglish
Pages (from-to)973-82
Number of pages10
JournalInternational journal of oncology
Volume36
Issue number4
DOIs
Publication statusPublished - Apr 2010

Keywords

  • Adult
  • Aged
  • Aged, 80 and over
  • Biological Markers
  • Cachexia
  • Case-Control Studies
  • Chromatography, Liquid
  • Electrophoresis, Polyacrylamide Gel
  • Esophageal Neoplasms
  • Female
  • Humans
  • Male
  • Middle Aged
  • Muscle Proteins
  • Predictive Value of Tests
  • Proteinuria
  • Proteomics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Stomach Neoplasms
  • Tandem Mass Spectrometry
  • Young Adult

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