Measuring the effects of fractionated radiation therapy in a 3D prostate cancer model system using SERS nanosensors

Victoria L. Camus, Grant Stewart, William H. Nailon, Duncan B. Mclaren, Colin J. Campbell

Research output: Contribution to journalArticlepeer-review

Abstract

Multicellular tumour spheroids (MTS) are three-dimensional cell cultures that possess their own microenvironments and provide a more meaningful model of tumour biology than monolayer cultures. As a result, MTS are becoming increasingly used as tumor models when measuring the efficiency of therapies. Monitoring the viability of live MTS is complicated by their 3D nature and conventional approaches such as fluorescence often require fixation and sectioning. In this paper we detail the use of Surface Enhanced Raman Spectroscopy (SERS) to measure the viability of MTS grown from prostate cancer (PC3) cells. Our results show that we can monitor loss of viability by measuring pH and redox potential in MTS and furthermore we demonstrate that SERS can be used to measure the effects of fractionation of a dose of radiotherapy in a way that has potential to inform treatment planning.
Original languageEnglish
Pages (from-to)5056-5061
Number of pages5
JournalAnalyst
DOIs
Publication statusPublished - 16 Jun 2016

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