Mechanical characterisation of unidirectional and cross-directional multilayered urinary bladder matrix (UBM) scaffolds

Anthony Callanan, Niall F Davis, Michael T Walsh, Timothy M McGloughlin

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Multilayered biological scaffolds derived from mammalian extracellular matrix (ECM) have shown promising long-term clinical results when reconstructing damaged tissues and organs. Despite their established clinical applicability, experimental studies that describe the effects of alternate manufacturing protocols on an ECM's mechanical properties are lacking. In the present study the mechanical properties of multilayered ‘unidirectional’ porcine urinary bladder matrix (UBM) scaffolds were determined in favour of its longitudinal and circumferential axes. The scaffold's unidirectional mechanical properties were then compared with ‘cross-directional’ UBM scaffolds. The results showed significant variations when alternate manufacturing protocols for multilayered UBM were applied. Unidirectional longitudinal UBM remained the strongest biomaterial on a consistent basis. Its failure strength occurred at 4.79 ± 0.85 MPa compared to 3.36 ± 0.53 MPa for unidirectional circumferential and 2.91 ± 1.05 MPa for cross-directional UBM respectively (p < 0.0001). Distensibility was greatest in unidirectional circumferential UBM with failure extension occurring at 14.77 ± 1.66 mm. In comparison, failure extension occurred at 12.88 ± 0.94 mm and 13.04 ± 4.35 mm for unidirectional longitudinal and cross-directional UBM respectively (p = 0.0024). The present study demonstrates that predefined manufacturing protocols for UBM should be considered when reconstructing anatomical structures with specific mechanical requirements.
Original languageEnglish
Pages (from-to)1368-1374
Number of pages7
JournalMedical Engineering and Physics
Volume34
Issue number9
DOIs
Publication statusPublished - Nov 2012

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