Mechanical Stimulation of Bone Cells Using Fluid Flow

Carmen Huesa, Astrid D. Bakker

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

Abstract / Description of output

This chapter describes several methods suitable for mechanically stimulating monolayers of bone cells by fluid shear stress (FSS) in vitro. Fluid flow is generated by pumping culture medium through two parallel plates, one of which contains a monolayer of cells. Methods for measuring nitric oxide production by bone cells in response to FSS are also described.
Original languageUndefined/Unknown
Title of host publicationBone Research Protocols, Second Edition
EditorsM. H. Helfrich, S. H. Ralston
Pages573-592
Number of pages20
Volume816
Edition2nd
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology

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