Mechanism of TrkB-mediated hippocampal long-term potentiation

Liliana Minichiello, Anna Maria Calella, Diego L Medina, Tobias Bonhoeffer, Rüdiger Klein, Martin Korte

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The TrkB receptor tyrosine kinase and its ligand, BDNF, have an essential role in certain forms of synaptic plasticity. However, the downstream pathways required to mediate these functions are unknown. We have studied mice with a targeted mutation in either the Shc or the phospholipase Cgamma (PLCgamma) docking sites of TrkB (trkB(SHC/SHC) and trkB(PLC/PLC) mice). We found that hippocampal long-term potentiation was impaired in trkB(PLC/PLC) mice, but not trkB(SHC/SHC) mice. BDNF stimulation of primary neurons derived from trkB(PLC/PLC) mice fully retained their ability to activate MAP kinases, whereas induction of CREB and CaMKIV phosphorylation was strongly impaired. The opposite effect was observed in trkB(SHC/SHC) neurons, suggesting that MAPKs and CREB act in parallel pathways. Our results provide genetic evidence that TrkB mediates hippocampal plasticity via recruitment of PLCgamma, and by subsequent phosphorylation of CaMKIV and CREB.
Original languageEnglish
Pages (from-to)121-37
Number of pages17
JournalNeuron
Volume36
Issue number1
Publication statusPublished - 2002

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