Abstract
Bacterial lipopolysaccharide (LPS) stably induced the protein kinase C substrate, MacMARCKS, in murine resident peritoneal macrophages; initial induction of MacMARCKS mRNA was detected within 15 min and was protein synthesis-independent. This response was observed in the macrophage cell line RAW264, and occurred also in response to plasmid DNA, a partial mimetic of other responses to LPS. In murine bone marrow-derived macrophages, MacMARCKS was expressed constitutively due to induction by macrophage colony-stimulating factor. Nuclear run-on transcription revealed that, like tumor necrosis factor alpha (TNF-alpha), MacMARCKS was transcribed constitutively in RAW264 cells. The MacMARCKS promoter was sequenced to -1.7 kb and the transcription start site determined. Transient transfections of RAW264 cells revealed that the 113-bp GC-rich proximal promoter contained all the elements required for both high basal activity and 15- to 20-fold activation by LPS.
Original language | English |
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Pages (from-to) | 528-34 |
Number of pages | 7 |
Journal | Journal of Leukocyte Biology |
Volume | 66 |
Issue number | 3 |
Publication status | Published - Sept 1999 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Base Sequence
- Bone Marrow Cells
- Gene Expression Regulation
- Gene Expression Regulation, Neoplastic
- Intracellular Signaling Peptides and Proteins
- Lipopolysaccharides
- Macrophages
- Macrophages, Peritoneal
- Membrane Proteins
- Mice
- Molecular Sequence Data
- Neoplasm Proteins
- Organ Specificity
- Promoter Regions, Genetic
- Protein Kinase C
- RNA, Messenger
- RNA, Neoplasm
- Recombinant Fusion Proteins
- Transcription, Genetic
- Transfection
- Tumor Cells, Cultured