Mechanisms of regulation of the MacMARCKS gene in macrophages by bacterial lipopolysaccharide

S Chang, K J Stacey, J Chen, E O Costelloe, A Aderem, D A Hume

Research output: Contribution to journalArticlepeer-review

Abstract

Bacterial lipopolysaccharide (LPS) stably induced the protein kinase C substrate, MacMARCKS, in murine resident peritoneal macrophages; initial induction of MacMARCKS mRNA was detected within 15 min and was protein synthesis-independent. This response was observed in the macrophage cell line RAW264, and occurred also in response to plasmid DNA, a partial mimetic of other responses to LPS. In murine bone marrow-derived macrophages, MacMARCKS was expressed constitutively due to induction by macrophage colony-stimulating factor. Nuclear run-on transcription revealed that, like tumor necrosis factor alpha (TNF-alpha), MacMARCKS was transcribed constitutively in RAW264 cells. The MacMARCKS promoter was sequenced to -1.7 kb and the transcription start site determined. Transient transfections of RAW264 cells revealed that the 113-bp GC-rich proximal promoter contained all the elements required for both high basal activity and 15- to 20-fold activation by LPS.
Original languageEnglish
Pages (from-to)528-34
Number of pages7
JournalJournal of Leukocyte Biology
Volume66
Issue number3
Publication statusPublished - Sep 1999

Keywords

  • Animals
  • Base Sequence
  • Bone Marrow Cells
  • Gene Expression Regulation
  • Gene Expression Regulation, Neoplastic
  • Intracellular Signaling Peptides and Proteins
  • Lipopolysaccharides
  • Macrophages
  • Macrophages, Peritoneal
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins
  • Organ Specificity
  • Promoter Regions, Genetic
  • Protein Kinase C
  • RNA, Messenger
  • RNA, Neoplasm
  • Recombinant Fusion Proteins
  • Transcription, Genetic
  • Transfection
  • Tumor Cells, Cultured

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