Mechanochemical Removal of Ribosome Biogenesis Factors from Nascent 60S Ribosomal Subunits

Cornelia Ulbrich, Meikel Diepholz, Jochen Bassler, Dieter Kressler, Brigitte Pertschy, Kyriaki Galani, Bettina Boettcher, Ed Hurt

Research output: Contribution to journalArticlepeer-review

Abstract

The dynein-related AAA ATPase Rea1 is a preribosomal factor that triggers an unknown maturation step in 60S subunit biogenesis. Using electron microscopy, we show that Rea1's motor domain is docked to the pre-60S particle and its tail-like structure, harboring a metal ion-dependent adhesion site (MIDAS), protrudes from the preribosome. Typically, integrins utilize a MIDAS to bind extracellular ligands, an interaction that is strengthened under applied tensile force. Likewise, the Rea1 MIDAS binds the preribosomal factor Rsa4, which is located on the pre-60S subunit at a site that is contacted by the flexible Rea1 tail. The MIDAS-Rsa4 interaction is essential for ATP-dependent dissociation of a group of non-ribosomal factors from the pre-60S particle. Thus, Rea1 aligns with its interacting partners on the preribosome to effect a necessary step on the path to the export-competent 60S subunit.

Original languageEnglish
Pages (from-to)911-922
Number of pages12
JournalCell
Volume138
Issue number5
DOIs
Publication statusPublished - 4 Sep 2009

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