Mesoscale standing wave imaging

Shannan Foylan; Jana Katharina Schniete; Lisa Sophie Kölln; John Dempster; Carsten Gram Hansen; Michael Shaw; Trevor John Bushell; Gail Mcconnell

doi:10.1111/jmi.13189

Mesoscale standing wave imaging

Shannan Foylan, Jana Katharina Schniete, Lisa Sophie Kölln, John Dempster, Carsten Gram Hansen, Michael Shaw, Trevor John Bushell, Gail Mcconnell

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Standing wave (SW) microscopy is a method that uses an interference pattern to excite fluorescence from labelled cellular structures and produces high-resolution images of three-dimensional objects in a two-dimensional dataset. SW microscopy is performed with high-magnification, high-numerical aperture objective lenses, and while this results in high-resolution images, the field of view is very small. Here we report upscaling of this interference imaging method from the microscale to the mesoscale using the Mesolens, which has the unusual combination of a low-magnification and high-numerical aperture. With this method, we produce SW images within a field of view of 4.4 mm × 3.0 mm that can readily accommodate over 16,000 cells in a single dataset. We demonstrate the method using both single-wavelength excitation and the multi-wavelength SW method TartanSW. We show application of the method for imaging of fixed and living cells specimens, with the first application of SW imaging to study cells under flow conditions.

Original language	English
Journal	Journal of Microscopy
Early online date	8 May 2023
DOIs	https://doi.org/10.1111/jmi.13189
Publication status	E-pub ahead of print - 8 May 2023

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https://onlinelibrary.wiley.com/doi/10.1111/jmi.13189

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@article{69fcb8bda4274110a16c2f823c566f81,

title = "Mesoscale standing wave imaging",

abstract = "Standing wave (SW) microscopy is a method that uses an interference pattern to excite fluorescence from labelled cellular structures and produces high-resolution images of three-dimensional objects in a two-dimensional dataset. SW microscopy is performed with high-magnification, high-numerical aperture objective lenses, and while this results in high-resolution images, the field of view is very small. Here we report upscaling of this interference imaging method from the microscale to the mesoscale using the Mesolens, which has the unusual combination of a low-magnification and high-numerical aperture. With this method, we produce SW images within a field of view of 4.4 mm × 3.0 mm that can readily accommodate over 16,000 cells in a single dataset. We demonstrate the method using both single-wavelength excitation and the multi-wavelength SW method TartanSW. We show application of the method for imaging of fixed and living cells specimens, with the first application of SW imaging to study cells under flow conditions.",

author = "Shannan Foylan and Schniete, {Jana Katharina} and K{\"o}lln, {Lisa Sophie} and John Dempster and Hansen, {Carsten Gram} and Michael Shaw and Bushell, {Trevor John} and Gail Mcconnell",

note = "Funding Information: This work was supported by the Biotechnology and Biological Sciences Research Council, grant numbers BB/P02565X/1 and BB/T011602/1. S.F. was supported by the Engineering and Physical Sciences Research Council iCASE studentship award, supported by the National Physical Laboratory. L.S.K. was supported by the Medical Research Council and Engineering and Physical Sciences Research Council Centre for Doctoral Training in Optical Medical Imaging, grant number EP/L016559/1. M.S. was supported by the National Measurement System funded by UK's Department for Business, Energy & Industrial Strategy. G.M. was partly supported by the Medical Research Council, grant number MR/K015583/1 and the Leverhulme Trust. Publisher Copyright: {\textcopyright} 2023 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society.",

year = "2023",

month = may,

day = "8",

doi = "10.1111/jmi.13189",

language = "English",

journal = "Journal of Microscopy",

issn = "0022-2720",

publisher = "Wiley-Blackwell",

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T1 - Mesoscale standing wave imaging

AU - Foylan, Shannan

AU - Schniete, Jana Katharina

AU - Kölln, Lisa Sophie

AU - Dempster, John

AU - Hansen, Carsten Gram

AU - Shaw, Michael

AU - Bushell, Trevor John

AU - Mcconnell, Gail

N1 - Funding Information: This work was supported by the Biotechnology and Biological Sciences Research Council, grant numbers BB/P02565X/1 and BB/T011602/1. S.F. was supported by the Engineering and Physical Sciences Research Council iCASE studentship award, supported by the National Physical Laboratory. L.S.K. was supported by the Medical Research Council and Engineering and Physical Sciences Research Council Centre for Doctoral Training in Optical Medical Imaging, grant number EP/L016559/1. M.S. was supported by the National Measurement System funded by UK's Department for Business, Energy & Industrial Strategy. G.M. was partly supported by the Medical Research Council, grant number MR/K015583/1 and the Leverhulme Trust. Publisher Copyright: © 2023 The Authors. Journal of Microscopy published by John Wiley & Sons Ltd on behalf of Royal Microscopical Society.

PY - 2023/5/8

Y1 - 2023/5/8

N2 - Standing wave (SW) microscopy is a method that uses an interference pattern to excite fluorescence from labelled cellular structures and produces high-resolution images of three-dimensional objects in a two-dimensional dataset. SW microscopy is performed with high-magnification, high-numerical aperture objective lenses, and while this results in high-resolution images, the field of view is very small. Here we report upscaling of this interference imaging method from the microscale to the mesoscale using the Mesolens, which has the unusual combination of a low-magnification and high-numerical aperture. With this method, we produce SW images within a field of view of 4.4 mm × 3.0 mm that can readily accommodate over 16,000 cells in a single dataset. We demonstrate the method using both single-wavelength excitation and the multi-wavelength SW method TartanSW. We show application of the method for imaging of fixed and living cells specimens, with the first application of SW imaging to study cells under flow conditions.

AB - Standing wave (SW) microscopy is a method that uses an interference pattern to excite fluorescence from labelled cellular structures and produces high-resolution images of three-dimensional objects in a two-dimensional dataset. SW microscopy is performed with high-magnification, high-numerical aperture objective lenses, and while this results in high-resolution images, the field of view is very small. Here we report upscaling of this interference imaging method from the microscale to the mesoscale using the Mesolens, which has the unusual combination of a low-magnification and high-numerical aperture. With this method, we produce SW images within a field of view of 4.4 mm × 3.0 mm that can readily accommodate over 16,000 cells in a single dataset. We demonstrate the method using both single-wavelength excitation and the multi-wavelength SW method TartanSW. We show application of the method for imaging of fixed and living cells specimens, with the first application of SW imaging to study cells under flow conditions.

U2 - 10.1111/jmi.13189

DO - 10.1111/jmi.13189

M3 - Article

SN - 0022-2720

JO - Journal of Microscopy

JF - Journal of Microscopy

ER -

Mesoscale standing wave imaging

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